Thrombin activity assay (ab234620)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture supernatant, Plasma, Serum
- Sensitivity: 0.031 AU/ml
Overview
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Product name
Thrombin activity assay
See all Thrombin kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Quantitative -
Sensitivity
0.031 AU/ml -
Range
0.05 AU/ml - 1.6 AU/ml -
Product overview
Thrombin Activity Assay Kit (ab234620) is developed to determine thrombin activity in human plasma, serum and cell culture samples. The amidolytic activity of thrombin is quantitated using a highly specific thrombin substrate releasing a pNA chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the thrombin enzymatic activity.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
Thrombin (activated factor II [IIa]) is a coagulation protein that has many effects in the coagulation cascade. Thrombin is a serine protease (EC 3.4.21.5) that converts soluble fibrinogen into insoluble strands of fibrin, as well as catalyzing many other coagulation-related reactions. Thrombin is in the form of alpha-thrombin that is the immediate end product of prothrombin activation: two further thrombin products can be identified, beta- and gamma- thrombin. These are degraded forms that may arise from autodigestion of a thrombin preparation.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 10X Diluent M Concentrate 1 x 20ml Human Thrombin Standard 1 vial Microplate 1 unit Sealing Tapes 3 units Thrombin Substrate 3 vials -
Research areas
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Function
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Factor II deficiency
Ischemic stroke
Thrombophilia due to thrombin defect
Pregnancy loss, recurrent, 2 -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). -
Cellular localization
Secreted, extracellular space. - Information by UniProt
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Alternative names
- Coagulation factor II
- Coagulation factor II thrombin
- EC 3.4.21.5
see all
Images
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Human Thrombin Chromogenic Activity Standard Curve
