Thrombin Inhibitor Screening Assay Kit (Fluorometric) (ab197007)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds, Plasma
Overview
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Product name
Thrombin Inhibitor Screening Assay Kit (Fluorometric)
See all Thrombin kits -
Detection method
Fluorescent -
Sample type
Plasma, Inhibitor compounds -
Assay type
Quantitative -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Thrombin Inhibitor Screening Assay Kit (Fluorometric) (ab197007) is an assay where the synthetic AMC-based peptide substrate provided is proteolytically cleaved by Thrombin in order to release a fluorophore, AMC, which can be easily quantified by fluorescence reader at Ex/Em = 350/450 nm. In the presence of Thrombin specific inhibitors, the extent of cleavage reaction is reduced or completely abolished. The loss in the fluorescence intensity can be correlated to the amount of inhibitor present in the assay solution.
The kit provides a simple and rapid method to screen potential inhibitors of thrombin and to characterize thrombin inhibitors found in plasma samples.
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Notes
Thrombin enzyme (Factor IIa) is an important clotting factor that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin inhibitors are used as anticoagulants to prevent arterial and venous thrombosis. Some of these inhibitors are currently in clinical use while others are in clinical development.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Thrombin Assay Buffer 1 x 15ml Thrombin Dilution Buffer 1 x 1ml Thrombin Enzyme 1 x 5µl Thrombin Inhibitor 1 x 10µl Thrombin Substrate 1 x 0.5ml -
Research areas
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Function
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Factor II deficiency
Ischemic stroke
Thrombophilia due to thrombin defect
Pregnancy loss, recurrent, 2 -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). -
Cellular localization
Secreted, extracellular space. - Information by UniProt
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Alternative names
- Coagulation factor II
- Coagulation factor II thrombin
- EC 3.4.21.5
see all
Images
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Thrombin Inhibitor Screening Assay Kit (Fluorometric) (ab197007)
Inhibition of thrombin activity by a thrombin Inhibitor (PPACK Dihydrochloride).
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Thrombin Inhibitor Screening Assay Kit (Fluorometric) (ab197007)Thrombin activity was measured in plasma samples in the presence and absence of Thrombin Inhibitor (PPACK Dihydrochloride). S = Substrate, I = Inhibitor, AB = Activation Buffer containing Factor Xa. Assays were performed following the kit protocol.
