Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SEK1MKK4S257-C5] to MKK4 (phospho S257)
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Human, African green monkey

Overview

  • Product name

    Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5]

  • Description

    Rabbit monoclonal [SEK1MKK4S257-C5] to MKK4 (phospho S257)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human MKK4 (phospho S257). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P45985

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: THP-1 (treated with sorbitol) cell extract; COS7 (treated with UV +TPA) cell extract. Flow cyt: NIH/3T3 cells (treated with pervanadate); HEK-293T cells (treated with UV + TPA).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Clonality

    Monoclonal

  • Clone number

    SEK1MKK4S257-C5

  • Isotype

    IgG

  • Light chain type

    kappa

Images

  • Flow Cytometry - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)
    Flow Cytometry - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)
    Flow cytometric analysis of 3T3 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using ab278619 at 0.1 µg/mL.
  • Western blot - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)
    Western blot - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)
    All lanes : Anti-RPS6 (phospho S240 + S244) antibody [SP45] (ab279619) at 0.05 µg/ml

    Lane 1 : COS7 cell extract untreated
    Lane 2 : COS7 cell extract treated with UV plus TPA
  • Flow Cytometry - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)
    Flow Cytometry - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)

    Flow cytometric analysis of HEK-293T cells treated with lambda phosphatase (red) or treated with UV+TPA (green) using ab278619, at 0.1 ug/mL,  or concentration-matched Rabbit (G9) mAb IgG Isotype Control for cells treated with phosphatase (black) or treated with UV+TPA (blue).

  • Western blot - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)
    Western blot - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)
    All lanes : Anti-RPS6 (phospho S240 + S244) antibody [SP45] (ab279619) at 0.05 µg/ml

    Lane 1 : THP1 cell extract treated with sorbitol
    Lane 2 : THP1 cell extract untreated
  • Western blot - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)
    Western blot - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)
    All lanes : Anti-RPS6 (phospho S240 + S244) antibody [SP45] (ab279619) at 0.05 µg/ml

    Lane 1 : THP1 cell extract treated with sorbitol
    Lane 2 : THP1 cell extract untreated
  • Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)
    Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)

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