Antibodies, Proteins, Kits and Reagents for Life Science

Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SEK1MKK4S257-C5] to MKK4 (phospho S257)
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Human, African green monkey

Product name

Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5]
Description

Rabbit monoclonal [SEK1MKK4S257-C5] to MKK4 (phospho S257)
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Mouse

Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human MKK4 (phospho S257). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P45985

Run BLAST with Run BLAST with
Positive control
- WB: THP-1 (treated with sorbitol) cell extract; COS7 (treated with UV +TPA) cell extract. Flow cyt: NIH/3T3 cells (treated with pervanadate); HEK-293T cells (treated with UV + TPA).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
Flow Cyt	Mouse Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

SEK1MKK4S257-C5
Isotype

IgG
Light chain type

kappa

Flow Cytometry - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)

Flow cytometric analysis of 3T3 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using ab278619 at 0.1 µg/mL.
Western blot - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)

All lanes : Anti-RPS6 (phospho S240 + S244) antibody [SP45] (ab279619) at 0.05 µg/ml

Lane 1 : COS7 cell extract untreated
Lane 2 : COS7 cell extract treated with UV plus TPA
Flow Cytometry - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)

Flow cytometric analysis of HEK-293T cells treated with lambda phosphatase (red) or treated with UV+TPA (green) using ab278619, at 0.1 ug/mL, or concentration-matched Rabbit (G9) mAb IgG Isotype Control for cells treated with phosphatase (black) or treated with UV+TPA (blue).
Western blot - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)

All lanes : Anti-RPS6 (phospho S240 + S244) antibody [SP45] (ab279619) at 0.05 µg/ml

Lane 1 : THP1 cell extract treated with sorbitol
Lane 2 : THP1 cell extract untreated
Western blot - Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)

All lanes : Anti-RPS6 (phospho S240 + S244) antibody [SP45] (ab279619) at 0.05 µg/ml

Lane 1 : THP1 cell extract treated with sorbitol
Lane 2 : THP1 cell extract untreated
Anti-MKK4 (phospho S257) antibody [SEK1MKK4S257-C5] (ab278619)

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