Anti-LAMP2 antibody [GL2A7] (ab13524)
![Anti-LAMP2 antibody [GL2A7] (ab13524)](https://www.abcam.com/ps/products/13/ab13524/Images/ab13524-397579-AntiLAMP2-antibody-GL2A7-western-blot-nih3t3-hek293-human-mouse.jpg "Anti-LAMP2 antibody [GL2A7] (ab13524)")
Key features and details
- Rat monoclonal [GL2A7] to LAMP2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-LAMP2 antibody [GL2A7]
See all LAMP2 primary antibodies -
Description
Rat monoclonal [GL2A7] to LAMP2 -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Tissue, cells or virus corresponding to Mouse LAMP2. Purified preparation of mouse liver lysosomal membranes.
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Positive control
- WB: HEK-293 and NIH/3T3 cell lysate.
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General notes
For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
GL2A7 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)All lanes : Anti-LAMP2 antibody [GL2A7] (ab13524) at 1/500 dilution (1 hr at RT)
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate at 20 µg
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) lysate at 10 µg
Secondary
All lanes : HRP Goat Anti-Rat, 1 hour at RT at 1/100 dilutionBlock: 5% milk + TBST for 1 hour at RT.
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![Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)](https://www.abcam.com/ps/products/13/ab13524/Images/ab13524-260757-anti-lamp2-antibody-gl2a7-western-blot.jpg)
Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524) This image is courtesy of an anonymous Abreview.Anti-LAMP2 antibody [GL2A7] (ab13524) at 1/1000 dilution + HEK293 cell lysate at 20 µg
Secondary
Goat anti-rat HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa (possible non-specific binding), 70 kDa (possible non-specific binding)
Exposure time: 3 minutesBlocked with 5% milk for 1 hour at RT.
Incubated with primary antibody in 5% BSA/TBST for 16 hours at 4°C.
