HRP Anti-HADHB antibody [5C3AB7] (ab198398)
![HRP Anti-HADHB antibody [5C3AB7] (ab198398)](https://www.abcam.com/ps/products/198/ab198398/Images/ab198398-238428-WBab1983981.jpg "HRP Anti-HADHB antibody [5C3AB7] (ab198398)")
Key features and details
- HRP Mouse monoclonal [5C3AB7] to HADHB
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-HADHB antibody [5C3AB7]
See all HADHB primary antibodies -
Description
HRP Mouse monoclonal [5C3AB7] to HADHB -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Positive control
- WB: HepG2 whole cell lysate.
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General notes
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
5C3AB7 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-HADHB antibody [5C3AB7] (ab198398)HRP Anti-HADHB antibody [5C3AB7] (ab198398) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198398 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
