Anti-Glutathione Reductase antibody [EPR7238] - BSA and Azide free (ab244233)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7238] to Glutathione Reductase - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Glutathione Reductase antibody [EPR7238] - BSA and Azide free
See all Glutathione Reductase primary antibodies -
Description
Rabbit monoclonal [EPR7238] to Glutathione Reductase - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab244233 is the carrier-free version of ab124995. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab244233 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7238 -
Isotype
IgG -
Research areas
Images
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This data was developed using ab124995, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Glutathione Reductase knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab124995 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab124995 was shown to specifically react with Glutathione Reductase when Glutathione Reductase knockout samples were used. Wild-type and Glutathione Reductase knockout samples were subjected to SDS-PAGE. ab124995 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Glutathione Reductase antibody [EPR7238] (ab124995) at 1/2000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab124995, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Glutathione Reductase antibody [EPR7238] (ab124995) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 56 kDaThis data was developed using ab124995, the same antibody clone in a different buffer formulation.
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All lanes : Anti-Glutathione Reductase antibody [EPR7238] (ab124995) at 1/2000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Predicted band size: 56 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab124995, the same antibody clone in a different buffer formulation.
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