Anti-HADHB antibody [5C3AB7] (ab110301)
![Anti-HADHB antibody [5C3AB7] (ab110301)](https://www.abcam.com/ps/products/110/ab110301/Images/ab110301-242584-anti-hadhb-antibody-5c3ab7-western-blot.jpg "Anti-HADHB antibody [5C3AB7] (ab110301)")
Key features and details
- Mouse monoclonal [5C3AB7] to HADHB
- Suitable for: IP, WB, Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-HADHB antibody [5C3AB7]
See all HADHB primary antibodies -
Description
Mouse monoclonal [5C3AB7] to HADHB -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC HumanIP HumanWB Human
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Immunogen
This information is considered to be commercially sensitive.
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Positive control
- HepG2 and HLM cell lysates; HDFn and HeLa cells.
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab110301 was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification. -
Clonality
Monoclonal -
Clone number
5C3AB7 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)Anti-HADHB antibody [5C3AB7] (ab110301) at 0.5 µg/ml (20 ug HepG2 cell lysate)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
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![Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)](https://www.abcam.com/ps/products/110/ab110301/Images/ab110301-123552-anti-hadhb-antibody-5c3ab7.gif)
Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)Anti-HADHB antibody [5C3AB7] (ab110301) at 0.5 µg/ml + HepG2 cell lysate
Predicted band size: 51 kDaAs a Western blot antibody, ab110301 works with HepG2 (human) cells.
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![Immunocytochemistry - Anti-HADHB antibody [5C3AB7] (ab110301)](https://www.abcam.com/ps/products/110/ab110301/Images/ab110301-123551-anti-hadhb-antibody-5c3ab7-immunocytochemistry.jpg)
Immunocytochemistry - Anti-HADHB antibody [5C3AB7] (ab110301)Immunocytochemistry analysis using ab110301 at 1µg/ml staining HADHB in HDFn cells (paraformaldehyde fixed and Triton X-100 permeabilized).
The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution. DAPI was used to stain the cell nuclei (blue). -
![Flow Cytometry - Anti-HADHB antibody [5C3AB7] (ab110301)](https://www.abcam.com/ps/products/110/ab110301/Images/ab110301-123550-anti-hadhb-antibody-5c3ab7-flow-cytometry.gif)
Flow Cytometry - Anti-HADHB antibody [5C3AB7] (ab110301)Flow cytometric analysis using ab110301 at 1µg/ml staining HADHB in HeLa cells (blue). Isotype control antibody (red). -
![Immunoprecipitation - Anti-HADHB antibody [5C3AB7] (ab110301)](https://www.abcam.com/ps/products/110/ab110301/Images/ab110301-123549-anti-hadhb-antibody-5c3ab7-immunoprecipitation.gif)
Immunoprecipitation - Anti-HADHB antibody [5C3AB7] (ab110301)As an IP antibody, MS733 works with human samples.
