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Signal Transduction Metabolism Mitochondrial

Alexa Fluor® 488 Anti-HADHB antibody [5C3AB7] (ab198592)

Alexa Fluor® 488 Anti-HADHB antibody [5C3AB7] (ab198592)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [5C3AB7] to HADHB
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG1

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Overview

  • Product name

    Alexa Fluor® 488 Anti-HADHB antibody [5C3AB7]
    See all HADHB primary antibodies
  • Description

    Alexa Fluor® 488 Mouse monoclonal [5C3AB7] to HADHB
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data
  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

     

     

     

     

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    ab110301 was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification.
  • Clonality

    Monoclonal
  • Clone number

    5C3AB7
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-HADHB antibody [5C3AB7] (ab198592)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-HADHB antibody [5C3AB7] (ab198592)

    ab198592 staining HADHB in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198592 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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