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Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (ab250143)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14238(B)] to PLP2 - BSA and Azide free
  • Suitable for: ICC, WB, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free
    See all PLP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14238(B)] to PLP2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250143 is the carrier-free version of ab180131 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250143 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (ab250143)
    Western blot - Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (ab250143)
    All lanes : Anti-PLP2 antibody [EPR14238(B)] (ab180131) at 1/5000 dilution

    Lane 1 : Human colon cancer tissue lysate
    Lane 2 : Human cerebellum tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 17 kDa



    This data was developed using ab180131, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (ab250143)
    Immunocytochemistry - Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (ab250143)
    This data was developed using ab180131, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of SW480 cells labeling PLP2 using ab180131 at 1/250 dilution. A Goat anti rabbit IgG (Dylight 488) at 1/250 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.
  • Immunoprecipitation - Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (ab250143)
    Immunoprecipitation - Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (ab250143)
    This data was developed using ab180131, the same antibody clone in a different buffer formulation.Immunoprecipitation analysis of Human colon cancer lysate labeling PLP2 using ab180131 at 1/30 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.
  • Flow Cytometry - Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (ab250143)
    Flow Cytometry - Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (ab250143)
    This data was developed using ab180131, the same antibody clone in a different buffer formulation.Flow cytometry analysis of U87-MG cells labeling PLP2 using ab180131 at 1/80 dilution. A Goat anti rabbit IgG (FITC) at 1/75 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit IgG.
  • Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (ab250143)
    Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (ab250143)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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