Anti-PP2A-2 catalytic subunit antibody - C-terminal (ab188253)
Key features and details
- Rabbit polyclonal to PP2A-2 catalytic subunit - C-terminal
- Suitable for: WB, ICC/IF, IP
- Reacts with: Schizosaccharomyces pombe
- Isotype: IgG
Overview
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Product name
Anti-PP2A-2 catalytic subunit antibody - C-terminal -
Description
Rabbit polyclonal to PP2A-2 catalytic subunit - C-terminal -
Host species
Rabbit -
Specificity
ab188253 recognized both PPA1 and PPA2 polypeptides in S. pombe because of their high amino acid similarity (~80% identity). -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Schizosaccharomyces pombe -
Immunogen
Recombinant fragment corresponding to Schizosaccharomyces pombe PPA2 (C terminal).
Database link: P23636 -
Positive control
- S. pombe cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PP2A-2 catalytic subunit antibody - C-terminal (ab188253)All lanes : Anti-PP2A-2 catalytic subunit antibody - C-terminal (ab188253) at 1/1000 dilution
Lane 1 : Wild-type S. pombe cell extract
Lane 2 : ΔPPA1
Lane 3 : ΔPPA2
Lane 4 : Wild-type carrying a multicopy plasmid with PPA1 gene
Lane 5 : Wild-type carrying a multicopy plasmid with PPA2 gene
Lane 6 : Wild-type carrying a multicopy plasmid with ADH promoter ligated with PPA2 gene
Predicted band size: 36 kDaThe positions of PPA1 (36 kDa) and PPA2 (39 kDa) polypeptide bands are indicated.
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Immunocytochemistry/ Immunofluorescence - Anti-PP2A-2 catalytic subunit antibody - C-terminal (ab188253)Immunofluorescence analysis of S.pombe cells, labeling PPA2 with ab188253 (right). DNA staining with DAPI (left). 1. ΔPPA2. 2. wild-type. 3. wild-type overexpressing PPA2 (nmt-PPA2, 18hr). Bar, 10 µm.