Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 10, 2021

Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13354] to ANP32C/PP32R1
Suitable for: WB, ICC/IF, Flow Cyt, IP
Reacts with: Human

Product name

Anti-ANP32C/PP32R1 antibody [EPR13354]
See all ANP32C/PP32R1 primary antibodies
Description

Rabbit monoclonal [EPR13354] to ANP32C/PP32R1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HUVEC, PC-3 and BxPC-3 cell lysates; MCF-7 cell lysate; PC-3 and MCF-7 cells.
General notes
This product was previously labelled as ANP32C

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13354
Isotype

IgG
Research areas

Western blot - Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242)

All lanes : Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242) at 1/10000 dilution

Lane 1 : HUVEC cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : BxPC-3 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1 & 3 : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Lane 2 : goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242)

Immunofluorescent analysis of 4% paraformaldehyde-fixed PC-3 cells labeling ANP32C/PP32R1 with ab181242 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Flow Cytometry - Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242)

Flow cytometric analysis of 2% paraformaldehyde-fixed MCF-7 cells labeling ANP32C/PP32R1 with ab181242 at 1/20 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Immunoprecipitation - Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242)

Western blot analysis of ANP32C/PP32R1 in MCF-7 cell lysate immunoprecipitated with ab181242 at 1/50 dilution.

Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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