Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13354] to ANP32C/PP32R1
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-ANP32C/PP32R1 antibody [EPR13354]
    See all ANP32C/PP32R1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13354] to ANP32C/PP32R1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HUVEC, PC-3 and BxPC-3 cell lysates; MCF-7 cell lysate; PC-3 and MCF-7 cells.

  • General notes

     

     

     This product was previously labelled as ANP32C

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13354

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242)
    Western blot - Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242)
    All lanes : Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242) at 1/10000 dilution

    Lane 1 : HUVEC cell lysate
    Lane 2 : PC-3 cell lysate
    Lane 3 : BxPC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1 & 3 : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
    Lane 2 : goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242)
    Immunocytochemistry/ Immunofluorescence - Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed PC-3 cells labeling ANP32C/PP32R1 with ab181242 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Flow Cytometry - Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242)
    Flow Cytometry - Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242)

    Flow cytometric analysis of 2% paraformaldehyde-fixed MCF-7 cells labeling ANP32C/PP32R1 with ab181242 at 1/20 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Immunoprecipitation - Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242)
    Immunoprecipitation - Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242)

    Western blot analysis of ANP32C/PP32R1 in MCF-7 cell lysate immunoprecipitated with ab181242 at 1/50 dilution.

    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242)
    Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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