Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921)
![Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921)](https://www.abcam.com/ps/products/189/ab189921/Images/ab189921-225712-anti-anp32c-antibody-epr13354-70r-western-blot.jpg "Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13354-70R] to ANP32C/PP32R1
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ANP32C/PP32R1 antibody [EPR13354-70R]
See all ANP32C/PP32R1 primary antibodies -
Description
Rabbit monoclonal [EPR13354-70R] to ANP32C/PP32R1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB RatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MCF-7, PC-3, BxPC-3, C6, Raw264.7 and NIH/3T3 cell lysates.
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General notes
This product was previously labelled as ANP32C
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13354-70R -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921)All lanes : Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921) at 1/10000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : BxPC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
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![Western blot - Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921)](https://www.abcam.com/ps/products/189/ab189921/Images/ab189921-225711-anti-anp32c-antibody-epr13354-70r-western-blot.jpg)
Western blot - Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921)All lanes : Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921) at 1/2000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : NIH/3T3 cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
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![Immunoprecipitation - Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921)](https://www.abcam.com/ps/products/189/ab189921/Images/ab189921-225710-anti-anp32c-antibody-epr13354-70r-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921)Western blot analysis of ANP32C/PP32R1 in MCF-7 cell lysate immunoprecipitated using ab189921 at 1/50 dilution (Lane1). Lane 2: PBS instead of MCF-7 lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
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![Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921)](https://www.abcam.com/ps/products/189/ab189921/Images/ab189921-4-benefits-of-recombinant-antibodies.png)
Anti-ANP32C/PP32R1 antibody [EPR13354-70R] (ab189921)
