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Human GLUL (Glutamine Synthetase) knockout HeLa cell pellet (ab278940)

Overview

  • Product name

    Human GLUL (Glutamine Synthetase) knockout HeLa cell pellet
    See all Glutamine Synthetase kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HeLa

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 8 bp deletion in exon 2.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.

  • Involvement in disease

    Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.

  • Sequence similarities

    Belongs to the glutamine synthetase family.

  • Developmental stage

    Expressed during early fetal stages.

  • Cellular localization

    Cytoplasm. Mitochondrion.
  • Information by UniProt

  • Alternative names

    • cell proliferation-inducing protein 59
    • Cgl2214
    • GLNA
    • GLNA_HUMAN
    • GLNS
    • GLUL
    • Glutamate ammonia ligase
    • Glutamate decarboxylase
    • Glutamate--ammonia ligase
    • glutamine synthase
    • Glutamine synthetase
    • glutamine synthetase I
    • GS
    • PIG 43
    • PIG 59
    • PIG43
    • PIG59
    • Proliferation inducing protein 43
    see all

Properties

Images

  • Western blot - Human GLUL (Glutamine Synthetase) knockout HeLa cell pellet (ab278940)
    Western blot - Human GLUL (Glutamine Synthetase) knockout HeLa cell pellet (ab278940)
    Lane 1: Wild-type HeLa cell lysate (40µg)

    Lane 2: GLUL knockout HeLa cell lysate (40µg)
    Lanes 1- 2: Merged signal (red and green). Green - ab197024 observed at 42 kDa. Red - loading control ab7291 observed at 50 kDa.
    ab197024 Anti-Glutamine Synthetase antibody [EPR16661] was shown to specifically react with Glutamine Synthetase in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261737 (knockout cell lysate ab256930) was used. Wild-type and Glutamine Synthetase knockout samples were subjected to SDS-PAGE. ab197024 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
  • Sanger Sequencing - Human GLUL (Glutamine Synthetase) knockout HeLa cell pellet (ab278940)
    Sanger Sequencing - Human GLUL (Glutamine Synthetase) knockout HeLa cell pellet (ab278940)

    Allele-1: 8 bp deletion in exon 2

     

  • Sanger Sequencing - Human GLUL (Glutamine Synthetase) knockout HeLa cell pellet (ab278940)
    Sanger Sequencing - Human GLUL (Glutamine Synthetase) knockout HeLa cell pellet (ab278940)

    Allele-2: 1 bp deletion in exon 2

     

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