Glutamine Assay Kit (Colorimetric) (ab197011)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 90 min
- Sample type: Other biological fluids, Plasma, Serum, Tissue, Urine
- Sensitivity: 25 µM
Overview
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Product name
Glutamine Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue -
Assay type
Quantitative -
Sensitivity
25 µM -
Assay time
0h 90m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Glutamine Assay kit (Colorimetric) ab197011 is a simple and sensitive assay that detects the biologically relevant concentrations of glutamine in biological fluids and tissues.
The glutamine assay protocol is based on the hydrolysis of Glutamine to Glutamate producing a stable colored signal (OD 450 nm), which is directly proportional to the amount of glutamine in the sample.
The assay can detect as little as 25 μM of glutamine in a variety of biological samples.
Glutamine assay protocol summary:
- add samples and standards to wells
- add hydrolysis mix and incubate for 30 min
- add reaction mix and incubate for 60 min
- analyze with a microplate reader. -
Notes
Previously called Glutamine Detection Assay Kit (Colorimetric).
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Developer 1 vial Development Buffer 1 x 25ml Development Enzyme Mix (lyophilized) Green 1 vial Glutamine Standard 1 vial Hydrolysis Buffer NM 1 x 25ml Hydrolysis Enzyme Mix 1 vial -
Research areas
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Relevance
Glutamine (abbreviated as Gln or Q; the abbreviation Glx or Z represents either glutamine or glutamic acid) is one of the 20 amino acids encoded by the standard genetic code. Its side chain is an amide formed by replacing the side-chain hydroxyl of glutamic acid with an amine functional group. It can therefore be considered the amide of glutamic acid. Its codons are CAA and CAG. Recent studies have proven its effectiveness in anabolic muscle growth from prolonged consumption. -
Alternative names
- Gln
- Q
Images
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Glutamine Standard Curve.
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Glutamine concentration in Human urine.Measurement of Glutamine concentration in Human urine (5 μl).
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Glutamine concentration in mouse tissues.Measurement of Glutamine concentration in Mouse tissues (brain [15 μg], liver [50 μg]). Samples were deproteinized using 10K Spin Column and spiked with known amount of Glutamine (6 nmol).