Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free (ab240193)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13022(B)] to Glutamine Synthetase - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free
    See all Glutamine Synthetase primary antibodies

  • Description

    Rabbit monoclonal [EPR13022(B)] to Glutamine Synthetase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver lysate, mouse and rat spleen lysate Jurkat, HAP1 and HeLa whole cell lysate (ab150035); IHC-P: Human glioma and liver tissues, mouse liver tissue; IHC-Fr: Rat and Mouse cerebrum.

  • General notes

    ab240193 is the carrier-free version of ab176562. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240193 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free (ab240193)
    Immunohistochemistry (Frozen sections) - Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free (ab240193)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilised mouse cerebrum tissue staining glutamine synthetase with ab176562 at 1/250 dilution, followed by alexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). DAPI was used as a nuclear counterstain.

    Positive staining on mouse cerebrum (PMID: 23895693).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176562).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free (ab240193)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free (ab240193)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human glioma tissue labeling Glutamine Synthetase with unpurified ab176562 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176562).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free (ab240193)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free (ab240193)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human glioma tissue sections labeling Glutamine Synthetase with purified ab176562 at 1:500 dilution (0.18 μg/ml). Heat mediated antigen retrieval was performed using citrate Buffer, pH6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176562).

  • Immunohistochemistry (Frozen sections) - Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free (ab240193)
    Immunohistochemistry (Frozen sections) - Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free (ab240193)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilised rat cerebrum tissue staining glutamine synthetase with ab176562 at 1/250 dilution, followed by alexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). DAPI was used as a nuclear counterstain.

    Positive staining on rat cerebrum (PMID: 23895693).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176562).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free (ab240193)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free (ab240193)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human liver tissue labeling Glutamine Synthetase with unpurified ab176562 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176562).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free (ab240193)
    Immunoprecipitation - Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free (ab240193)

    Western blot analysis on Immunoprecipitation pellet from either 1) Human fetal liver lysate, or 2) 1xPBS (negative control); showing Glutamine Synthetase, with unpurified ab176562, diluted in 1% BSA, at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176562).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free (ab240193)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free (ab240193) This image is courtesy of Alex Van Engelenburg

    ab176562 staining Glutamine Synthetasein Mouse Liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, blocked with PB ab64226 for 10 minutes at room temperature and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/200) for 30 minutes. A HRP-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176562).

  • Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free (ab240193)
    Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free (ab240193)

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