Anti-DNA polymerase eta antibody (ab234855)
Key features and details
- Rabbit polyclonal to DNA polymerase eta
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DNA polymerase eta antibody
See all DNA polymerase eta primary antibodies -
Description
Rabbit polyclonal to DNA polymerase eta -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human DNA polymerase eta aa 400-650.
Database link: Q9Y253 -
Positive control
- WB: A549 and K562 cell lysate. IHC-P: Human tonsil tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DNA polymerase eta antibody (ab234855)All lanes : Anti-DNA polymerase eta antibody (ab234855) at 1/500 dilution
Lane 1 : A549 (Human lung carcinoma cell line) cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 78 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-DNA polymerase eta antibody (ab234855)HepG2 (human liver hepatocellular carcinoma cell line) cells labeling DNA polymerase eta using ab234855 at 1/100 dilution in ICC/IF. Secondary antibody was an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA polymerase eta antibody (ab234855)Paraffin-embedded human tonsil tissue stained for DNA polymerase eta using ab234855 at 1/100 dilution in immunohistochemical analysis.
