Anti-DNA polymerase eta antibody (ab236450)
Key features and details
- Rabbit polyclonal to DNA polymerase eta
- Suitable for: WB, IP, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DNA polymerase eta antibody
See all DNA polymerase eta primary antibodies -
Description
Rabbit polyclonal to DNA polymerase eta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human DNA polymerase eta (C terminal). (Around 300 amino acids of the C-terminus of the human protein).
Database link: Q9Y253 -
Positive control
- WB: U-2 OS, MEF and INS-1 whole cell extracts. IP: U-2 OS whole cell extract. Flow cyt: U-2 OS cells. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DNA polymerase eta antibody (ab236450)All lanes : Anti-DNA polymerase eta antibody (ab236450) at 1/2000 dilution
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) treated with control siRNA, whole cell lysate
Lane 2 : U-2 OS treated with DNA polymerase eta siRNA, whole cell lysate
Lane 3 : MEF (mouse embryonic fibroblast cell line) whole cell lysate
Lane 4 : INS-1 (rat pancreatic beta cell line) whole cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 78 kDaBlocking buffer: 5% milk in TBS-T
Incubated overnight with primary antibody.
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Immunoprecipitation - Anti-DNA polymerase eta antibody (ab236450)DNA polymerase eta was immunoprecipitated from ~400 µg U-2 OS (human bone osteosarcoma epithelial cell line) whole cell extract with 3 μl ab236450. Western blot was performed from the immunoprecipitate using ab236450 at 1/2000 dilution.
Lane 1: U-2 OS whole cell extract (Input)
Lane 2: Control IgG IP in U-2 OS whole cell extract.
Lane 3: ab236450 IP in U-2 OS whole cell extract.
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Flow Cytometry - Anti-DNA polymerase eta antibody (ab236450)Flow cytometric analysis of 1% PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cell line labeling DNA polymerase eta with ab236450 at 1/200 dilution (green) compared with a no primary antibody control (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-DNA polymerase eta antibody (ab236450)U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for DNA polymerase eta (green) using ab236450 at 1/2000 dilution in ICC/IF (green). The nuclear counter stain is DAPI (blue). The right-hand panel shows the merged images.
