Anti-Adiponectin Receptor 2/ADIPOR2 antibody (ab77612)
Key features and details
- Goat polyclonal to Adiponectin Receptor 2/ADIPOR2
- Suitable for: WB, IHC-P, ICC, Flow Cyt
- Reacts with: Mouse, Rat, Human, Macaque monkey
- Isotype: IgG
Overview
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Product name
Anti-Adiponectin Receptor 2/ADIPOR2 antibody
See all Adiponectin Receptor 2/ADIPOR2 primary antibodies -
Description
Goat polyclonal to Adiponectin Receptor 2/ADIPOR2 -
Host species
Goat -
Specificity
Recognizes Human, Rat, Mouse and Macaca ADPR2 Receptor. May cross react with ADPR1 Receptor. -
Tested applications
Suitable for: WB, IHC-P, ICC, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Macaque monkey
Predicted to work with: Chicken, Cow, Pig, Xenopus laevis
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
This antibody may be used in developing sandwich ELISA assays in combination with antibodies specific to N terminus of ADPR2 Receptor
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
This antibody may be used in developing sandwich ELISA assays in combination with antibodies specific to N terminus of ADPR2 Receptor -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Images
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Western blot - Anti-Adiponectin Receptor 2/ADIPOR2 antibody (ab77612)Anti-Adiponectin Receptor 2/ADIPOR2 antibody (ab77612) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin Receptor 2/ADIPOR2 antibody (ab77612)Paraffin section of human liver tissue stained with antibody to human ADPR2 receptor C-terminus ab77612 at 1/250 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin Receptor 2/ADIPOR2 antibody (ab77612)Paraffin section of human muscle tissue stained with antibody to human ADPR2 receptor C-terminus ab77612 at 1/250 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin Receptor 2/ADIPOR2 antibody (ab77612) This image is courtesy of an anonymous AbreviewAb77612 staining Adiponectin receptor 2/ADIPOR2 in Human liver tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using citric acid. Samples were fixed with paraformaldehyde and blocked with 10% normal goat serum for 15 minutes at 25°C. Samples were incubated with primary antibody for 12 hours at 4°C. A HRP conjugated goat anti-rabbit IgG was used as a secondary antibody at 10µg/ml.
