Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)

Key features and details

  • Mouse monoclonal [1E1-E8-H4] to beta Tubulin - Microtubule Marker
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker
    See all beta Tubulin primary antibodies

  • Description

    Mouse monoclonal [1E1-E8-H4] to beta Tubulin - Microtubule Marker

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Rat
    Human
    African green monkey
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human beta Tubulin.
    Database link: P07437

  • Positive control

    • A431, HeLa and Jurkat cell lysates.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 17.08.2018.  Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)
    Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)
    All lanes : Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205) at 1/5000 dilution

    Lane 1 : C6 Cell lysate
    Lane 2 : K562 Cell lysate
    Lane 3 : COS7 Cell lysate
    Lane 4 : 3T3 Cell lysate
    Lane 5 : HeLa Cell lysate

    Predicted band size: 49 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205) This image is courtesy of an Abreview submitted by Justin G Lichter

    ab131205 staining beta Tubulin (red) in dog left ventricular cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 10% normal goat serum for 1 hour at 27°C. Samples were incubated with primary antibody (1/200 in PBS + 2% BSA + 2% NGS) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG monoclonal (1/100) was used as the secondary antibody. Green indicates cell membrane with wheat germ agglutinin (WGA) conjugated to Alexa Fluor® 555. This image was produced with antibody produced in ascites. 

    See Abreview

  • Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)
    Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)
    All lanes : Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205) at 1/500 dilution

    Lane 1 : COS7 cell lysate
    Lane 2 : LNCap cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : C2C12 cell lysate

    Predicted band size: 49 kDa



    This image was produced with antibody produced in ascites. 

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)

    Immunocytochemistry stain of Hela using ab131205(1:300). This image was produced with antibody produced in ascites. 

  • Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)
    Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)

    ab131205 stability test under 37ºC for 50 hours compared to 4°C for 50 hours. This image was produced with antibody produced in ascites. 

  • Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)
    Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)
    All lanes : Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Predicted band size: 49 kDa
    Observed band size: 49 kDa



    This image was produced with antibody produced in ascites. 

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