Anti-Tubulin antibody [DM1A +DM1B] - Loading Control (ab44928)
![Anti-Tubulin antibody [DM1A +DM1B] - Loading Control (ab44928)](https://www.abcam.com/ps/products/44/ab44928/Images/ab44928-57106-anti-tubulin-antibody-dm1a-dm1b-loading-control-western-blot.jpg "Anti-Tubulin antibody [DM1A +DM1B] - Loading Control (ab44928)")
Key features and details
- Mouse monoclonal [DM1A +DM1B] to Tubulin - Loading Control
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Rat, Human, Drosophila melanogaster
- Isotype: IgG1
Overview
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Product name
Anti-Tubulin antibody [DM1A +DM1B] - Loading Control
See all Tubulin primary antibodies -
Description
Mouse monoclonal [DM1A +DM1B] to Tubulin - Loading Control -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Tissue/ cell preparation, Native chick brain microtubules.
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Epitope
aa 426-450 -
Positive control
- In Western Blot, this antibody gave a positive signal in the following whole cell lysates: HeLa; NIH3T3; PC12. Ls174T, MAD109 cells. Skin or lung.
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General notes
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
DM1A +DM1B -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Tubulin antibody [DM1A +DM1B] - Loading Control (ab44928) Image courtesy of Qifeng Lin by Abreview.All lanes : Anti-Tubulin antibody [DM1A +DM1B] - Loading Control (ab44928) at 1/5000 dilution
All lanes : Whole cell lysate prepared from human colon cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP sheep anti-mouse polyclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tubulin antibody [DM1A +DM1B] - Loading Control (ab44928)](https://www.abcam.com/ps/products/44/ab44928/Images/ab44928-57108-anti-tubulin-antibody-dm1a-dm1b-loading-control-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tubulin antibody [DM1A +DM1B] - Loading Control (ab44928)Ab44928 staining human normal placenta. Staining is localized to the cytoplamsm
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
![Western blot - Anti-Tubulin antibody [DM1A +DM1B] - Loading Control (ab44928)](https://www.abcam.com/ps/products/44/ab44928/Images/ab44928-57105-anti-tubulin-antibody-dm1a-dm1b-loading-control-western-blot.jpg)
Western blot - Anti-Tubulin antibody [DM1A +DM1B] - Loading Control (ab44928)All lanes : Anti-Tubulin antibody [DM1A +DM1B] - Loading Control (ab44928) at 5 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
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![Flow Cytometry - Anti-Tubulin antibody [DM1A +DM1B] - Loading Control (ab44928)](https://www.abcam.com/ps/products/44/ab44928/Images/ab44928-57104-anti-tubulin-antibody-dm1a-dm1b-loading-control-flow-cytometry.jpg)
Flow Cytometry - Anti-Tubulin antibody [DM1A +DM1B] - Loading Control (ab44928)Overlay histogram showing HeLa cells stained with ab44928 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab44928, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
