Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3973] to Trefoil Factor 3
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Trefoil Factor 3 antibody [EPR3973]
    See all Trefoil Factor 3 primary antibodies

  • Description

    Rabbit monoclonal [EPR3973] to Trefoil Factor 3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human uterus cancer and colon cancer lysates; Human colon tissue.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR3973

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104)

    Immunohistochemical analysis of Trefoil Factor 3 in paraffin-embedded Human colon tissue using ab109104 at 1/500 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104)
    Western blot - Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104)
    All lanes : Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104) at 1/1000 dilution

    Lane 1 : Human uterus cancer lysate
    Lane 2 : Human colon cancer lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 9 kDa

  • Flow Cytometry - Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104)
    Flow Cytometry - Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104)

    Flow Cytometry analysis of HT-29 (human colorectal adenocarcinoma) cells labeling Trefoil Factor 3 with unpurified ab109104 at 1/100 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104)
    Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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