Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸ 10 µl 127 315 ₸

Order now and get it on Thursday March 04, 2021

Anti-delta Sarcoglycan antibody [EPR8706] (ab137101)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8706] to delta Sarcoglycan
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-delta Sarcoglycan antibody [EPR8706]
See all delta Sarcoglycan primary antibodies
Description

Rabbit monoclonal [EPR8706] to delta Sarcoglycan
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human delta Sarcoglycan aa 200-300. The exact sequence is proprietary.
Positive control
- Human fetal heart and skeletal muscle lysates; Human heart tissue. IP: Human skeletal muscle lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8706
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta Sarcoglycan antibody [EPR8706] (ab137101)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labelling delta Sarcoglycan with ab137101 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-delta Sarcoglycan antibody [EPR8706] (ab137101)

All lanes : Anti-delta Sarcoglycan antibody [EPR8706] (ab137101) at 1/1000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human skeletal muscle lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 32 kDa
Observed band size: 35 kDa
why is the actual band size different from the predicted?
Immunoprecipitation - Anti-delta Sarcoglycan antibody [EPR8706] (ab137101)

Purified ab137101 at 1/20 dilution (1µg) immunoprecipitating delta Sarcoglycan in Human skeletal muscle lysate.
Lane 1 (input): Human skeletal muscle lysate 10µg
Lane 2 (+): ab137101 + Human skeletal muscle lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137101 in Human skeletal muscle lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 35 kDa
Anti-delta Sarcoglycan antibody [EPR8706] (ab137101)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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