Anti-delta Sarcoglycan antibody (ab115019)
Key features and details
- Goat polyclonal to delta Sarcoglycan
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-delta Sarcoglycan antibody
See all delta Sarcoglycan primary antibodies -
Description
Goat polyclonal to delta Sarcoglycan -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide:
C-RLPHGSYTPTGTR
, corresponding to internal sequence amino acids 245-257 of Human delta Sarcoglycan(NP_000328.2; NP_758447.1; NP_001121681.1). -
Positive control
- Human and Rat heart lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab115019 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-delta Sarcoglycan antibody (ab115019)Anti-delta Sarcoglycan antibody (ab115019) at 0.1 µg/ml + Human heart lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta Sarcoglycan antibody (ab115019)ab115019 (3.8µg/ml) staining of paraffin embedded Human Skeletal Muscle shows sarcolemmar staining of the fibres. Tissue treated: Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
