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Anti-GNLY/Granulysin antibody [EPR22110-128] - BSA and Azide free (ab238460)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22110-128] to GNLY/Granulysin - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-GNLY/Granulysin antibody [EPR22110-128] - BSA and Azide free
    See all GNLY/Granulysin primary antibodies

  • Description

    Rabbit monoclonal [EPR22110-128] to GNLY/Granulysin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: NK-92 cells.

  • General notes

    ab238460 is the carrier-free version of ab223326 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab238460 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as GNLY

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-GNLY/Granulysin antibody [EPR22110-128] - BSA and Azide free (ab238460)
    Immunoprecipitation - Anti-GNLY/Granulysin antibody [EPR22110-128] - BSA and Azide free (ab238460)

    GNLY/Granulysin was immunoprecipitated from 0.35 mg of NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate with ab223326 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223326 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: NK-92 whole cell lysate 10 μg (Input).
    Lane 2: ab223326 IP in NK-92 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223326 in NK-92 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    The doublets likely represent the two isoforms of GNLY/Granulysin according to the UniProt Database.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223326).

  • Flow Cytometry - Anti-GNLY/Granulysin antibody [EPR22110-128] - BSA and Azide free (ab238460)
    Flow Cytometry - Anti-GNLY/Granulysin antibody [EPR22110-128] - BSA and Azide free (ab238460)

    Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween 20-permeabilized PBMCs (human peripheral blood mononuclear cell) cells labeling GNLY/Granulysin with ab223326 at 1/600 (right) compared with a Isotype control details (ab172730) (left).  

    Human PBMCs were stained with PE-conjugated anti-human CD56.
    Cells were then fixed with 2% PFA for 10min and permeabilized with 0.1% Tween-20, followed by intracellular staining with rabbit IgG (ab172730, left) or ab223326, right).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223326).

  • Immunocytochemistry/ Immunofluorescence - Anti-GNLY/Granulysin antibody [EPR22110-128] - BSA and Azide free (ab238460)
    Immunocytochemistry/ Immunofluorescence - Anti-GNLY/Granulysin antibody [EPR22110-128] - BSA and Azide free (ab238460)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) cells labeling GNLY/Granulysin with ab223326 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the NK-92 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Negative control: K-562 (PMID: 2434598).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223326).

  • Anti-GNLY/Granulysin antibody [EPR22110-128] - BSA and Azide free (ab238460)
    Anti-GNLY/Granulysin antibody [EPR22110-128] - BSA and Azide free (ab238460)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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