Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 02, 2021

Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22110-101] to GNLY/Granulysin
Suitable for: ICC/IF, IHC-P, WB
Reacts with: Human

Product name

Anti-GNLY/Granulysin antibody [EPR22110-101]
See all GNLY/Granulysin primary antibodies
Description

Rabbit monoclonal [EPR22110-101] to GNLY/Granulysin
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged human GNLY/Granulysin recombinant protein; NK-92 whole cell lysate. IHC-P: Human endometrium and spleen tissue. ICC/IF: NK-92 and human PBMC cells.
General notes
This product was previously labelled as GNLY.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22110-101
Isotype

IgG
Research areas

Western blot - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)

All lanes : Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333) at 1/1000 dilution

Lane 1 : NK-92 (human malignant non-hodgkin's lymphoma natural killer cell) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 16 kDa

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 103 seconds

The doublets represent two isoforms of GNLY/Granulysin according to UniProt Database.

Negative control: K562 cell line (PMID:2434598).

This blot was developed using a higher sensitivity ECL substrate.
Immunocytochemistry/ Immunofluorescence - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PBMC (human peripheral blood mononuclear cell) cells labeling GNLY/Granulysin with ab241333 at 1/250 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary antibody (ab150077) (Green) at 1/1000 dilution.

Confocal image showing cytoplasmic staining in a part of human PBMC. The nuclear counterstain was DAPI (Blue). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) was used as a counterstain (Red).
Immunocytochemistry/ Immunofluorescence - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) cells labeling GNLY/Granulysin with ab241333 at 1/250 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary antibody (ab150077) (Green) at 1/1000 dilution.

Confocal image showing cytoplasmic staining in NK-92 cells. The nuclear counterstain was DAPI (Blue). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) was used as a counterstain (Red).
Western blot - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)

Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333) at 1/1000 dilution + His-tagged human GNLY/Granulysin recombinant protein (aa23-145) 10 ng

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 16 kDa

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 15 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling GNLY/Granulysin with ab241333 at 1/4000 dilution, followed by a ready to use Goat anti-Rabbit IgG H&L (HRP). The section was incubated with ab for 30 mins at 37°C. Sporadic staining on human spleen (PMID: 30151671) is observed, performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)

Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling GNLY/Granulysin with ab241333 at 1/4000 dilution, followed by a ready to use Goat anti-Rabbit IgG H&L (HRP). The section was incubated with ab for 30 mins at 37°C. Positive staining on lymphocytes of human endometrium (PMID: 26488136) is observed, performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins.
Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)

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