Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)
![Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)](https://www.abcam.com/ps/products/241/ab241333/Images/ab241333-323602-anti-gnly-antibody-epr22110-101-western-blot.jpg "Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22110-101] to GNLY/Granulysin
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-GNLY/Granulysin antibody [EPR22110-101]
See all GNLY/Granulysin primary antibodies -
Description
Rabbit monoclonal [EPR22110-101] to GNLY/Granulysin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: His-tagged human GNLY/Granulysin recombinant protein; NK-92 whole cell lysate. IHC-P: Human endometrium and spleen tissue. ICC/IF: NK-92 and human PBMC cells.
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General notes
This product was previously labelled as GNLY.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22110-101 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)All lanes : Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333) at 1/1000 dilution
Lane 1 : NK-92 (human malignant non-hodgkin's lymphoma natural killer cell) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 16 kDaBlocking/Diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 103 seconds
The doublets represent two isoforms of GNLY/Granulysin according to UniProt Database.
Negative control: K562 cell line (PMID:2434598).
This blot was developed using a higher sensitivity ECL substrate.
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![Immunocytochemistry/ Immunofluorescence - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)](https://www.abcam.com/ps/products/241/ab241333/Images/ab241333-323604-anti-gnly-antibody-epr22110-101-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PBMC (human peripheral blood mononuclear cell) cells labeling GNLY/Granulysin with ab241333 at 1/250 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) (Green) at 1/1000 dilution.
Confocal image showing cytoplasmic staining in a part of human PBMC. The nuclear counterstain was DAPI (Blue). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as a counterstain (Red).
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![Immunocytochemistry/ Immunofluorescence - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)](https://www.abcam.com/ps/products/241/ab241333/Images/ab241333-323603-anti-gnly-antibody-epr22110-101-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) cells labeling GNLY/Granulysin with ab241333 at 1/250 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) (Green) at 1/1000 dilution.
Confocal image showing cytoplasmic staining in NK-92 cells. The nuclear counterstain was DAPI (Blue). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as a counterstain (Red).
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![Western blot - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)](https://www.abcam.com/ps/products/241/ab241333/Images/ab241333-323601-anti-gnly-antibody-epr22110-101-western-blot.jpg)
Western blot - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333) at 1/1000 dilution + His-tagged human GNLY/Granulysin recombinant protein (aa23-145) 10 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 16 kDaBlocking/Diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 15 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)](https://www.abcam.com/ps/products/241/ab241333/Images/ab241333-2-241333IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling GNLY/Granulysin with ab241333 at 1/4000 dilution, followed by a ready to use Goat anti-Rabbit IgG H&L (HRP). The section was incubated with ab for 30 mins at 37°C. Sporadic staining on human spleen (PMID: 30151671) is observed, performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)](https://www.abcam.com/ps/products/241/ab241333/Images/ab241333-323599-anti-gnly-antibody-epr22110-101-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling GNLY/Granulysin with ab241333 at 1/4000 dilution, followed by a ready to use Goat anti-Rabbit IgG H&L (HRP). The section was incubated with ab for 30 mins at 37°C. Positive staining on lymphocytes of human endometrium (PMID: 26488136) is observed, performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins.
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![Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)](https://www.abcam.com/ps/products/241/ab241333/Images/ab241333-7-benefits-of-recombinant-antibodies.png)
Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333)
