Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-GNLY/Granulysin antibody [EPR22110-128] (ab223326)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22110-128] to GNLY/Granulysin
Suitable for: WB, ICC/IF, IP, Flow Cyt
Reacts with: Human

Product name

Anti-GNLY/Granulysin antibody [EPR22110-128]
See all GNLY/Granulysin primary antibodies
Description

Rabbit monoclonal [EPR22110-128] to GNLY/Granulysin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged human GNLY/Granulysin recombinant protein (aa23-145) ; NK-92 whole cell lysate. IP: NK-92 whole cell lysate. ICC/IF: NK-92 cells. Flow cyt: PBMCs.
General notes
This product was previously labelled as GNLY

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22110-128
Isotype

IgG
Research areas

Western blot - Anti-GNLY/Granulysin antibody [EPR22110-128] (ab223326)

All lanes : Anti-GNLY/Granulysin antibody [EPR22110-128] (ab223326) at 1/1000 dilution

Lane 1 : NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 16 kDa
Observed band size: 13,14 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.

The doublets likely represent the two isoforms of GNLY/Granulysin according to the UniProt Database.

Negative control: K-562 cell line (PMID:2434598).

This blot was developed using high sensitivity ECL substrate.
Flow Cytometry - Anti-GNLY/Granulysin antibody [EPR22110-128] (ab223326)

Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween 20-permeabilized PBMCs (human peripheral blood mononuclear cell) cells labeling GNLY/Granulysin with ab223326 at 1/600 (right) compared with a Isotype control details (ab172730) (left).

Human PBMCs were stained with PE-conjugated anti-human CD56.
Cells were then fixed with 2% PFA for 10min and permeabilized with 0.1% Tween-20, followed by intracellular staining with rabbit IgG (ab172730, left) or ab223326, right).
Immunoprecipitation - Anti-GNLY/Granulysin antibody [EPR22110-128] (ab223326)

GNLY/Granulysin was immunoprecipitated from 0.35 mg of NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate with ab223326 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223326 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: NK-92 whole cell lysate 10 μg (Input).
Lane 2: ab223326 IP in NK-92 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223326 in NK-92 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.

The doublets likely represent the two isoforms of GNLY/Granulysin according to the UniProt Database.
Immunocytochemistry/ Immunofluorescence - Anti-GNLY/Granulysin antibody [EPR22110-128] (ab223326)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) cells labeling GNLY/Granulysin with ab223326 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the NK-92 cell line is observed. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Negative control: K-562 (PMID: 2434598).
Western blot - Anti-GNLY/Granulysin antibody [EPR22110-128] (ab223326)

Anti-GNLY/Granulysin antibody [EPR22110-128] (ab223326) at 1/1000 dilution + His-tagged human GNLY/Granulysin recombinant protein (aa23-145) at 0.01 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 16 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds

Blocking and dilution buffer: 5% NFDM/TBST.
Anti-GNLY/Granulysin antibody [EPR22110-128] (ab223326)

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