Recombinant Mouse CD8 alpha + CD8B protein (Tagged) (ab277035)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant Mouse CD8 alpha + CD8B protein (Tagged) -
Purity
> 95 % SDS-PAGE. -
Endotoxin level
Expression system
HEK 293 cellsAccession
Protein length
Protein fragmentAnimal free
NoNature
Recombinant-
Amino Acid Sequence 1
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Species
Mouse -
Sequence
MASPLTRFLS LNLLLLGESI ILGSGEAKPQ APELRIFPKK MDAELGQKVD LVCEVLGSVS QGCSWLFQNS SSKLPQPTFV VYMASSHNKI TWDEKLNSSK LFSAMRDTNN KYVLTLNKFS KENEGYYFCS VISNSVMYFS SVVPVLQKVN STTTKPVLRT PSPVHPTGTS QPQRPEDCRP RGSVKGTGLD FACDIY -
Predicted molecular weight
24 kDa including tags -
Molecular weight information
The recombinant heterodimer of mouse CD8A&CD8B comprises 405 (210+195) amino acids and has a calculated molecular mass of 45.9 (23.7+22.2) KDa. -
Amino acids
1 to 196 -
Additional sequence information
FLAG tag at C-terminus.
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Amino Acid Sequence 2
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Species
Mouse -
Sequence
MQPWLWLVFS MKLAALWSSS ALIQTPSSLL VQTNHTAKMS CEVKSISKLT SIYWLRERQD PKDKYFEFLA SWSSSKGVLY GESVDKKRNI ILESSDSRRP FLSIMNVKPE DSDFYFCATV GSPKMVFGTG TKLTVVDVLP TTAPTKKTTL KMKKKKQCPF PHPETQKGLT CSLTT -
Predicted molecular weight
22 kDa including tags -
Amino acids
1 to 175 -
Tags
His tag C-Terminus
Specifications
Our Abpromise guarantee covers the use of ab277035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 100% PBS -
ReconstitutionThis information is lot specific. Please contact our technical Support team for details.
General Info
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Cellular localization
CD8 alpha: Secreted and Cell membrane. CD8B: Isoform 1: Cell membrane; Single-pass type I membrane protein (Probable). Isoform 2: Cell membrane; Single-pass type I membrane protein (Probable). Isoform 3: Secreted (Probable). Isoform 4: Cell membrane; Single-pass type I membrane protein (Probable). Isoform 5: Cell membrane; Single-pass type I membrane protein (Probable). Isoform 6: Secreted (Probable). Isoform 7: Secreted (Probable). Isoform 8: Secreted (Probable).
Images
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SDS-PAGE - Recombinant Mouse CD8 alpha + CD8B protein (ab277035)SDS-PAGE analysis of ab277035
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab277035 has not yet been referenced specifically in any publications.
Preparation and Storage
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Cellular localization
CD8 alpha: Secreted and Cell membrane. CD8B: Isoform 1: Cell membrane; Single-pass type I membrane protein (Probable). Isoform 2: Cell membrane; Single-pass type I membrane protein (Probable). Isoform 3: Secreted (Probable). Isoform 4: Cell membrane; Single-pass type I membrane protein (Probable). Isoform 5: Cell membrane; Single-pass type I membrane protein (Probable). Isoform 6: Secreted (Probable). Isoform 7: Secreted (Probable). Isoform 8: Secreted (Probable).
Images
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SDS-PAGE - Recombinant Mouse CD8 alpha + CD8B protein (ab277035)SDS-PAGE analysis of ab277035