Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free (ab250935)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15552] to ROR beta/RORB - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free
    See all ROR beta/RORB primary antibodies

  • Description

    Rabbit monoclonal [EPR15552] to ROR beta/RORB - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human ROR beta/RORB aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q92753

  • Positive control

    • WB: HepG2 whole cell lysate (ab7900) and human cerebellum tissue lysate; ICC: Mouse primary neural/glia cells.

  • General notes

    ab250935 is the carrier-free version of ab187657 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250935 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as ROR beta

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free (ab250935)
    Western blot - Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free (ab250935)
    Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657) at 1/1000 dilution + HepG2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa



    This data was developed using ab187657, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free (ab250935)
    Immunocytochemistry - Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free (ab250935)

    This data was developed using ab187657, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cells labelling ROR beta/RORB with ab187657 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing cytoplamic staining in astrocytes.Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution (2 µg/mL).

     

  • Immunocytochemistry - Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free (ab250935)
    Immunocytochemistry - Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free (ab250935)

    This data was developed using ab187657, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural / glia cells labelling ROR beta/RORB with ab187657 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing cytoplasmic staining in mouse primary astrocyte. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution (2 µg/mL).

  • Western blot - Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free (ab250935)
    Western blot - Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free (ab250935)
    Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657) at 1/1000 dilution + Human cerebellum tissue lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 53 kDa



    This data was developed using ab187657, the same antibody clone in a different buffer formulation.

     

  • Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free (ab250935)
    Anti-ROR beta/RORB antibody [EPR15552] - BSA and Azide free (ab250935)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.