Anti-ADAR1 antibody (ab226188)
Key features and details
- Rabbit polyclonal to ADAR1
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ADAR1 antibody
See all ADAR1 primary antibodies -
Description
Rabbit polyclonal to ADAR1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human ADAR1 aa 950-1000. The exact sequence is proprietary. (NP_001102.2).
Database link: P55265 -
Positive control
- IHC-P: Human ovarian carcinoma tissue. WB: HEK-293T, HeLa and Jurkat whole cell lysate (ab7899). IP: HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226188 was affinity purified using an epitope specific to ADAR1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ADAR1 antibody (ab226188)All lanes : Anti-ADAR1 antibody (ab226188) at 0.4 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 136 kDa
Exposure time: 10 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAR1 antibody (ab226188)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for ADAR1 using ab226188 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
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Immunoprecipitation - Anti-ADAR1 antibody (ab226188)ADAR1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226188 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226188 at 1 µg/ml.
Lane 1: ab226188 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
