Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)
![Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)](https://www.abcam.com/ps/products/184/ab184962/Images/ab184962-217711-anti-pcbp2-hnrnp-e2-antibody-epr14858-western-blot.jpg "Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14858] to PCBP2/hnRNP E2
- Suitable for: Flow Cyt, IP, ICC/IF, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-PCBP2/hnRNP E2 antibody [EPR14858]
See all PCBP2/hnRNP E2 primary antibodies -
Description
Rabbit monoclonal [EPR14858] to PCBP2/hnRNP E2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanIP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Raji and K562 cell lysates; Human clear cell carcinoma of kidney tissue; Human skin tissue; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14858 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)All lanes : Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Raji cell lysate
Lane 3 : K562 cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 39 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)](https://www.abcam.com/ps/products/184/ab184962/Images/ab184962-217710-anti-pcbp2-hnrnp-e2-antibody-epr14858-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)Immunohistochemical analysis of paraffin-embedded human skin tissue labeling PCBP2/hnRNP E2 with ab184962 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)](https://www.abcam.com/ps/products/184/ab184962/Images/ab184962-217709-anti-pcbp2-hnrnp-e2-antibody-epr14858-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney tissue labeling PCBP2/hnRNP E2 with ab184962 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)](https://www.abcam.com/ps/products/184/ab184962/Images/ab184962-217708-anti-pcbp2-hnrnp-e2-antibody-epr14858-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling PCBP2/hnRNP E2 with ab184962 at 1/250 dilution (red) followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
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![Flow Cytometry - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)](https://www.abcam.com/ps/products/184/ab184962/Images/ab184962-217707-anti-pcbp2-hnrnp-e2-antibody-epr14858-flow-cytometry.jpg)
Flow Cytometry - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)Flow cytometric anlaysis of HeLa cells (2% paraformaldehyde-fixed) labeling PCBP2/hnRNP E2 with ab184962 at 1/110 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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![Immunoprecipitation - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)](https://www.abcam.com/ps/products/184/ab184962/Images/ab184962-217706-anti-pcbp2-hnrnp-e2-antibody-epr14858-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)Immunoprecipitation analysis of HeLa lysates labeling PCBP2/hnRNP E2 with ab18492 at 1/50 dilution (Lane 1) and PBS (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG used as secondary antibody.
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![Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)](https://www.abcam.com/ps/products/184/ab184962/Images/ab184962-7-benefits-of-recombinant-antibodies.png)
Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)
