Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCBP2/hnRNP E2 antibody [EPR14858] - BSA and Azide free (ab236137)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14858] to PCBP2/hnRNP E2 - BSA and Azide free
  • Suitable for: IHC-P, IP, ICC/IF, Flow Cyt, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-PCBP2/hnRNP E2 antibody [EPR14858] - BSA and Azide free
    See all PCBP2/hnRNP E2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14858] to PCBP2/hnRNP E2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human skin tissue.

  • General notes

    Ab236137 is the carrier-free version of ab184962. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236137 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] - BSA and Azide free (ab236137)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] - BSA and Azide free (ab236137)

    Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney tissue labeling PCBP2/hnRNP E2 with ab184962 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184962).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-PCBP2/hnRNP E2 antibody [EPR14858] - BSA and Azide free (ab236137)
    Immunocytochemistry/ Immunofluorescence - Anti-PCBP2/hnRNP E2 antibody [EPR14858] - BSA and Azide free (ab236137)

    Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling PCBP2/hnRNP E2 with ab184962 at 1/250 dilution (red) followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184962).

  • Flow Cytometry - Anti-PCBP2/hnRNP E2 antibody [EPR14858] - BSA and Azide free (ab236137)
    Flow Cytometry - Anti-PCBP2/hnRNP E2 antibody [EPR14858] - BSA and Azide free (ab236137)

    Flow cytometric anlaysis of HeLa cells (2% paraformaldehyde-fixed) labeling PCBP2/hnRNP E2 with ab184962 at 1/110 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184962).

  • Immunoprecipitation - Anti-PCBP2/hnRNP E2 antibody [EPR14858] - BSA and Azide free (ab236137)
    Immunoprecipitation - Anti-PCBP2/hnRNP E2 antibody [EPR14858] - BSA and Azide free (ab236137)

    Immunoprecipitation analysis of HeLa lysates labeling PCBP2/hnRNP E2 with ab18492 at 1/50 dilution (Lane 1) and PBS (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG used as secondary antibody. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184962).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] - BSA and Azide free (ab236137)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] - BSA and Azide free (ab236137)

    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling PCBP2/hnRNP E2 with ab184962 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184962).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-PCBP2/hnRNP E2 antibody [EPR14858] - BSA and Azide free (ab236137)
    Anti-PCBP2/hnRNP E2 antibody [EPR14858] - BSA and Azide free (ab236137)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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