All lanes : Anti-BANF1/BAF antibody [EPR7669] (ab129074) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 10 kDa
Observed band size: 10 kDa

This data was developed using ab129074, the same antibody clone in a different buffer formulation.

This data was developed using ab129074, the same antibody clone in a different buffer formulation.Overlay histogram showing HepG2 cells stained with ab129074 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129074, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1?g/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

This data was developed using ab129074, the same antibody clone in a different buffer formulation.ab129074, at 1/100 dilution, staining BANF1/BAF in paraffin-embedded Human thyroid gland carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.