Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-USP28 antibody [EPR4249(2)] - BSA and Azide free (ab225537)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4249(2)] to USP28 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-USP28 antibody [EPR4249(2)] - BSA and Azide free
    See all USP28 primary antibodies

  • Description

    Rabbit monoclonal [EPR4249(2)] to USP28 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293T, HeLa, HT1376, SW480 and A431 cell lysates; Human heart tissue lysate; HeLa cells.

  • General notes

    Ab225537 is the carrier-free version of ab126604. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab225537 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-USP28 antibody [EPR4249(2)] - BSA and Azide free (ab225537)
    Western blot - Anti-USP28 antibody [EPR4249(2)] - BSA and Azide free (ab225537)

    This WB data was generated using the same anti-USP28 antibody clone [EPR4249(2)] in a different buffer formulation (cat# ab126604).

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: USP28 knockout HAP1 cell lysate (20 µg) 
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab126604 observed at 128 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab126604 was shown to recognize USP28 when USP28 knockout samples were used, along with additional cross-reactive bands. Wild-type and USP28 knockout samples were subjected to SDS-PAGE. ab126604 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-USP28 antibody [EPR4249(2)] - BSA and Azide free (ab225537)
    Flow Cytometry - Anti-USP28 antibody [EPR4249(2)] - BSA and Azide free (ab225537)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling USP28  with unpurified ab126604 at 1:200 dilution(1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126604).

  • Immunocytochemistry/ Immunofluorescence - Anti-USP28 antibody [EPR4249(2)] - BSA and Azide free (ab225537)
    Immunocytochemistry/ Immunofluorescence - Anti-USP28 antibody [EPR4249(2)] - BSA and Azide free (ab225537)
    ab126604 at 1/50 dilution staining USP28 in HeLa cells by Immunofluorescence.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126604).

  • OI-RD Scanning - Anti-USP28 antibody [EPR4249(2)] - BSA and Azide free (ab225537)
    OI-RD Scanning - Anti-USP28 antibody [EPR4249(2)] - BSA and Azide free (ab225537)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126604).

  • Anti-USP28 antibody [EPR4249(2)] - BSA and Azide free (ab225537)
    Anti-USP28 antibody [EPR4249(2)] - BSA and Azide free (ab225537)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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