Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-USP28 antibody [EPR4250(2)] (ab110744)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4250(2)] to USP28
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-USP28 antibody [EPR4250(2)]
    See all USP28 primary antibodies

  • Description

    Rabbit monoclonal [EPR4250(2)] to USP28

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293T, SW480, HeLa, and A431 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)
    Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: USP28 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lanes 1 - 3: Merged signal (red and green). Green - ab110744 observed at 128 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab110744 was shown to specifically react with USP28 when USP28 knockout samples were used, along with additional cross-reactive bands. Wild-type and USP28 knockout samples were subjected to SDS-PAGE.  Ab110744 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)
    Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)
    All lanes : Anti-USP28 antibody [EPR4250(2)] (ab110744) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : SW480 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 122 kDa

  • Anti-USP28 antibody [EPR4250(2)] (ab110744)
    Anti-USP28 antibody [EPR4250(2)] (ab110744)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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