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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Deubiquitination

Anti-USP28 antibody (ab188240)

Anti-USP28 antibody (ab188240)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to USP28
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-USP28 antibody
    See all USP28 primary antibodies
  • Description

    Rabbit polyclonal to USP28
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human USP28 aa 1-400.
    Database link: Q96RU2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • U2OS, NIH 3T3 and INS-1 cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Deubiquitination
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Other
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Deubiquitination
    • Cancer
    • Cell Death
    • Apoptosis
    • Other

Images

  • Western blot - Anti-USP28 antibody (ab188240)
    Western blot - Anti-USP28 antibody (ab188240)
    All lanes : Anti-USP28 antibody (ab188240) at 1/2000 dilution

    Lane 1 : Lysate from U2OS cells transfected with a control si
    Lane 2 : Lysate from U2OS cells transfected with a USP28 si
    Lane 3 : NIH 3T3 cell lysate
    Lane 4 : INS-1 cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 122 kDa

  • Immunoprecipitation - Anti-USP28 antibody (ab188240)
    Immunoprecipitation - Anti-USP28 antibody (ab188240)

    Western blot analysis (using ab188240) of immunoprecipitates from U2OS cell extracts

    Lane 1: Input

    Lane 2: immunoprecipitate with control serum+protein A sepharose.

    Lane 3: immunoprecipitate with ab188240 + protein A sepharose.

  • Immunocytochemistry/ Immunofluorescence - Anti-USP28 antibody (ab188240)
    Immunocytochemistry/ Immunofluorescence - Anti-USP28 antibody (ab188240)

    Immunofluorescent analysis of 3% PFA-fixed U2OS cells transfected with either a control si (left) or USP28 si (right) labeling USP28 with ab188240 at 1/2000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-USP28 antibody (ab188240)

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    Anti-USP28 antibody (ab70893)

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    Anti-USP28 antibody [EPR4250(2)] (ab110744)

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