Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-USP28 antibody [EPR4249(2)] (ab126604)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4249(2)] to USP28
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-USP28 antibody [EPR4249(2)]
    See all USP28 primary antibodies

  • Description

    Rabbit monoclonal [EPR4249(2)] to USP28

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide, corresponding to residues in Human USP28.

  • Positive control

    • 293T, HeLa, HT1376, SW480 and A431 cell lysates; Human heart tissue lysate; HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-USP28 antibody [EPR4249(2)] (ab126604)
    Western blot - Anti-USP28 antibody [EPR4249(2)] (ab126604)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: USP28 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab126604 observed at 128 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab126604 was shown to recognize USP28 when USP28 knockout samples were used, along with additional cross-reactive bands. Wild-type and USP28 knockout samples were subjected to SDS-PAGE. ab126604 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-USP28 antibody [EPR4249(2)] (ab126604)
    Immunocytochemistry/ Immunofluorescence - Anti-USP28 antibody [EPR4249(2)] (ab126604)
    ab126604 at 1/50 dilution staining USP28 in HeLa cells by Immunofluorescence.
  • Flow Cytometry - Anti-USP28 antibody [EPR4249(2)] (ab126604)
    Flow Cytometry - Anti-USP28 antibody [EPR4249(2)] (ab126604)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling USP28  with unpurified ab126604 at 1:200 dilution(1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-USP28 antibody [EPR4249(2)] (ab126604)
    Western blot - Anti-USP28 antibody [EPR4249(2)] (ab126604)
    All lanes : Anti-USP28 antibody [EPR4249(2)] (ab126604) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HT1376 cell lysate
    Lane 4 : Human heart tissue lysate
    Lane 5 : SW480 cell lysate
    Lane 6 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 122 kDa

  • OI-RD Scanning - Anti-USP28 antibody [EPR4249(2)] (ab126604)
    OI-RD Scanning - Anti-USP28 antibody [EPR4249(2)] (ab126604)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-USP28 antibody [EPR4249(2)] (ab126604)
    Anti-USP28 antibody [EPR4249(2)] (ab126604)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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