Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15943] to USP14/TGT - C-terminal
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-USP14/TGT antibody [EPR15943] - C-terminal
See all USP14/TGT primary antibodies -
Description
Rabbit monoclonal [EPR15943] to USP14/TGT - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB RatHuman -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HCT-116, HeLa, Jurkat, 293T, C6 and RAW 264.7 cell lysates; HeLa and Jurkat cells.
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General notes
This product was previously labelled as USP14
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15943 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618) at 1/1000 dilution
All lanes : HAP1 cell line
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : donkey anti-rabbit IgG-HRP at 1/3000 dilution
Predicted band size: 56 kDa
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Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling USP14/TGT with ab192618 at 1/70 dilution (5 μg/ml). A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Counterstain: DAPI.
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Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling USP14/TGT with ab192618 at 1/70 dilution (red) or a rabbit IgG (negative) (black), unlabeled cells (blue) followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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All lanes : Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HCT-116 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 56 kDa
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All lanes : Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618) at 1/2000 dilution
Lane 1 : C6 cell lysate
Lane 2 : RAW 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 56 kDa
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