Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Wednesday March 10, 2021

Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15943] to USP14/TGT - C-terminal
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-USP14/TGT antibody [EPR15943] - C-terminal
See all USP14/TGT primary antibodies
Description

Rabbit monoclonal [EPR15943] to USP14/TGT - C-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HCT-116, HeLa, Jurkat, 293T, C6 and RAW 264.7 cell lysates; HeLa and Jurkat cells.
General notes
This product was previously labelled as USP14

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15943
Isotype

IgG
Research areas

Western blot - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618) This image is courtesy of an anonymous Abreview.

All lanes : Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618) at 1/1000 dilution

All lanes : HAP1 cell line

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : donkey anti-rabbit IgG-HRP at 1/3000 dilution

Predicted band size: 56 kDa

See Abreview
Immunocytochemistry/ Immunofluorescence - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)

Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling USP14/TGT with ab192618 at 1/70 dilution (5 μg/ml). A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Counterstain: DAPI.
Flow Cytometry - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)

Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling USP14/TGT with ab192618 at 1/70 dilution (red) or a rabbit IgG (negative) (black), unlabeled cells (blue) followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Western blot - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)

All lanes : Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618) at 1/2000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HCT-116 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 56 kDa
Western blot - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)

All lanes : Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618) at 1/2000 dilution

Lane 1 : C6 cell lysate
Lane 2 : RAW 264.7 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 56 kDa
Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com