Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C14orf169 / NO66 antibody [EPR18754] (ab194292)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18754] to C14orf169 / NO66
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-C14orf169 / NO66 antibody [EPR18754]
    See all C14orf169 / NO66 primary antibodies

  • Description

    Rabbit monoclonal [EPR18754] to C14orf169 / NO66

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal brain and fetal kidney lysates; HEK293, U-2 OS, A549, NCCIT, MCF7 cell lysates. IHC: Human kidney tissue. IP: HEK293 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-C14orf169 / NO66 antibody [EPR18754] (ab194292)
    Immunoprecipitation - Anti-C14orf169 / NO66 antibody [EPR18754] (ab194292)

    Immunoprecipitation of C14orf169 / NO66 from 1mg of HEK293 (Human epithelial cells from embryonic kidney) whole cell lysate with  ab194292 at 1/1000 dilution. Western blot was performed from the immunoprecipitate using ab194292 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 was used for detection.
    Lane 1: HEK293 whole cell lysate 10µg (Input).
    Lane 2: ab194292 IP in HEK293 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab194292 in HEK293 whole cell lysates.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 second.

  • Western blot - Anti-C14orf169 / NO66 antibody [EPR18754] (ab194292)
    Western blot - Anti-C14orf169 / NO66 antibody [EPR18754] (ab194292)
    All lanes : Anti-C14orf169 / NO66 antibody [EPR18754] (ab194292) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 71 kDa


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C14orf169 / NO66 antibody [EPR18754] (ab194292)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C14orf169 / NO66 antibody [EPR18754] (ab194292)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling C14orf169 / NO66 with ab194292 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on epithelial cells of human colon. Counter-stained with Hematoxylin.
    Secondary antibody only control: PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-C14orf169 / NO66 antibody [EPR18754] (ab194292)
    Western blot - Anti-C14orf169 / NO66 antibody [EPR18754] (ab194292)
    Lanes 1-4 : Anti-C14orf169 / NO66 antibody [EPR18754] (ab194292) at 1/1000 dilution
    Lane 5 : Anti-C14orf169 / NO66 antibody [EPR18754] (ab194292) at 1/5000 dilution

    Lane 1 : HEK293 (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 2 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma) whole cell lysate
    Lane 4 : NCCIT (human pluripotent embryonic carcinoma) whole cell lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 71 kDa


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and diluting buffer.

  • Anti-C14orf169 / NO66 antibody [EPR18754] (ab194292)
    Anti-C14orf169 / NO66 antibody [EPR18754] (ab194292)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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