Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Thursday March 04, 2021

Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18656] to C14orf169 / NO66
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-C14orf169 / NO66 antibody [EPR18656]
See all C14orf169 / NO66 primary antibodies
Description

Rabbit monoclonal [EPR18656] to C14orf169 / NO66
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HEK-293, U-2 OS, NCCIT, F9, LLC, mESC,C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal liver lysate; Mouse lung lysate. IP: HEK-293 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IP	Human
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18656
Isotype

IgG
Research areas

Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861)

All lanes : Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861) at 1/1000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 4 : NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate
Lane 5 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
Lane 6 : LLC (Mouse lung carcinoma cell line) whole cell lysate
Lane 7 : mESC (Mouse embryonic stem cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 71 kDa
Observed band size: 71 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
The predicted MW of human’s, mouse’s and rat’s NO66 are 71kDa, 68kDa and 67kDa, respectively.
Immunoprecipitation - Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861)

C14orf169 / NO66 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate with ab192861 at 1/50 dilution.

Western blot was performed from the immunoprecipitate using ab192861 at 1/1000 dilution.

VeriBlot for IP Detection Reagent(HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HEK-293 whole cell lysate, 10µg (Input).

Lane 2: ab192861 IP in HEK-293 whole cell lysate.

Lane 3: Rabbit IgG,monoclonal [EPR18656]-Isotype Control (ab172730) instead of ab192861 in HEK-293 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861)

All lanes : Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861) at 1/1000 dilution

Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 71 kDa
Observed band size: 71 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861)

All lanes : Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861) at 1/200 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : Mouse lung lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 71 kDa
Observed band size: 71 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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