Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18656] to C14orf169 / NO66
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-C14orf169 / NO66 antibody [EPR18656]
    See all C14orf169 / NO66 primary antibodies

  • Description

    Rabbit monoclonal [EPR18656] to C14orf169 / NO66

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293, U-2 OS, NCCIT, F9, LLC, mESC,C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal liver lysate; Mouse lung lysate. IP: HEK-293 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861)
    Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861)
    All lanes : Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate
    Lane 4 : NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate
    Lane 5 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
    Lane 6 : LLC (Mouse lung carcinoma cell line) whole cell lysate
    Lane 7 : mESC (Mouse embryonic stem cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 71 kDa
    Observed band size: 71 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The predicted MW of human’s, mouse’s and rat’s NO66 are 71kDa, 68kDa and 67kDa, respectively.

  • Immunoprecipitation - Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861)
    Immunoprecipitation - Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861)

    C14orf169 / NO66 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate with ab192861 at 1/50 dilution.

    Western blot was performed from the immunoprecipitate using ab192861 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent(HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293 whole cell lysate, 10µg (Input).

    Lane 2: ab192861 IP in HEK-293 whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR18656]-Isotype Control (ab172730) instead of ab192861 in HEK-293 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861)
    Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861)
    All lanes : Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 71 kDa
    Observed band size: 71 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861)
    Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861)
    All lanes : Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861) at 1/200 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Mouse lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 71 kDa
    Observed band size: 71 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861)
    Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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