Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BANF1/BAF antibody [EPR7668] (ab129184)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7668] to BANF1/BAF
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Dog, Human

Overview

  • Product name

    Anti-BANF1/BAF antibody [EPR7668]
    See all BANF1/BAF primary antibodies

  • Description

    Rabbit monoclonal [EPR7668] to BANF1/BAF

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    Human
    IHC-P
    Human
    WB
    Dog
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human BANF1/BAF. The exact sequence is proprietary.
    Database link: O75531

  • Positive control

    • WB: HeLa, HepG2, MDCK, and HEK-293T cell lysates. IHC-P: Human colonic carcinoma, and ovarian carcinoma tissues. ICC/IF: HeLa, HepG2 and NIH/3T3 cells. Flow Cyt: HeLa cells.

  • General notes

     

     

     This product was previously labelled as BANF1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Dissociation constant (KD)

    KD = 2.18 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR7668

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] (ab129184)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] (ab129184)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue sections labeling BANF1/BAF with Purified ab129184 at 1:100 dilution (0.78 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] (ab129184)
    Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] (ab129184)

    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling BANF1/BAF with Purified ab129184 at 1:100 dilution (7.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-BANF1/BAF antibody [EPR7668] (ab129184)
    Western blot - Anti-BANF1/BAF antibody [EPR7668] (ab129184)
    Anti-BANF1/BAF antibody [EPR7668] (ab129184) at 1/5000 dilution + MDCK (NBL-2) (Dog kidney epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 10 kDa
    Observed band size: 10 kDa



    Blocking Buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-BANF1/BAF antibody [EPR7668] (ab129184)
    Western blot - Anti-BANF1/BAF antibody [EPR7668] (ab129184)
    All lanes : Anti-BANF1/BAF antibody [EPR7668] (ab129184) at 1/1000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 10 kDa
    Observed band size: 10 kDa



    Blocking Buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-BANF1/BAF antibody [EPR7668] (ab129184)
    Western blot - Anti-BANF1/BAF antibody [EPR7668] (ab129184)
    All lanes : Anti-BANF1/BAF antibody [EPR7668] (ab129184) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysates
    Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 10 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] (ab129184)
    Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] (ab129184)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryo fibroblast cells)cells labeling BANF1/BAF with ab129184 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing mainly nuclear with cytoplasmic staining on NIH/3T3 cell line. The nuclear counterstain is DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] (ab129184)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] (ab129184) This image is courtesy of an anonymous Abreview

    ab129184 staining BANF1/BAF in MDCK (Canine kidney cell line) cell pellets in paraffin by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Cell pellets were fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 1% serum for 2 hours at room temperature; antigen retrieval was by heat mediation in Tris/EDTA pH 9. Samples were incubated with primary antibody (1/100 in 1% BSA + 1% FBS) for 16 hours. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Flow Cytometry - Anti-BANF1/BAF antibody [EPR7668] (ab129184)
    Flow Cytometry - Anti-BANF1/BAF antibody [EPR7668] (ab129184)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling BANF1/BAF (red) with ab129184 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] (ab129184)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] (ab129184)

    ab129184, at a 1/100 dilution, staining BANF1/BAF in paraffin embedded Human colonic carcinoma tissue by Immunohistochemistry.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] (ab129184)
    Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] (ab129184)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma) cells labeling BANF1/BAF with ab129184 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing mainly nuclear with weakly cytoplasmic staining on Hela cell line. The nuclear counterstain is DAPI (blue).

  • OI-RD Scanning - Anti-BANF1/BAF antibody [EPR7668] (ab129184)
    OI-RD Scanning - Anti-BANF1/BAF antibody [EPR7668] (ab129184)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-BANF1/BAF antibody [EPR7668] (ab129184)
    Anti-BANF1/BAF antibody [EPR7668] (ab129184)

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