Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16649] to HMGA1 - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-HMGA1 antibody [EPR16649] - BSA and Azide free
    See all HMGA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16649] to HMGA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251363 is the carrier-free version of ab202070. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251363 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)
    Western blot - Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)
    All lanes : Anti-HMGA1 antibody [EPR16649] (ab202070) at 1/10000 dilution

    Lane 1 : NCCIT (Human pluripotent embryonal carcinoma) whole cell lysate
    Lane 2 : BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 4 : SK-OV-3 (Human ovarian cancer cell line) whole cell lysate
    Lane 5 : Caco-2 (Human colorectal adenocarcinoma cells) whole cell lysate
    Lane 6 : Human testis tissue lysate
    Lane 7 : Human lung tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 20 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab202070, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)
    Immunocytochemistry - Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)
    This data was developed using ab202070, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling HMGA1 with ab202070 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on BxPC-3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:
    -ve control 1: ab202070 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
  • Flow Cytometry - Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)
    Flow Cytometry - Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)
    This data was developed using ab202070, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HMGA1 with ab202070 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
  • Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)
    Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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