Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)
![Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)](https://www.abcam.com/ps/products/251/ab251363/Images/ab251363-408215-anti-hmga1-antibody-epr16649-bsa-and-azide-free-western-blot.jpg "Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16649] to HMGA1 - BSA and Azide free
- Suitable for: ICC, WB, Flow Cyt
- Reacts with: Human
Overview
-
Product name
Anti-HMGA1 antibody [EPR16649] - BSA and Azide free
See all HMGA1 primary antibodies -
Description
Rabbit monoclonal [EPR16649] to HMGA1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab251363 is the carrier-free version of ab202070. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251363 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR16649 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)All lanes : Anti-HMGA1 antibody [EPR16649] (ab202070) at 1/10000 dilution
Lane 1 : NCCIT (Human pluripotent embryonal carcinoma) whole cell lysate
Lane 2 : BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 4 : SK-OV-3 (Human ovarian cancer cell line) whole cell lysate
Lane 5 : Caco-2 (Human colorectal adenocarcinoma cells) whole cell lysate
Lane 6 : Human testis tissue lysate
Lane 7 : Human lung tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?This data was developed using ab202070, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Immunocytochemistry - Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)](https://www.abcam.com/ps/products/251/ab251363/Images/ab251363-2-anti-hmga1-antibody-epr16649-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)This data was developed using ab202070, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling HMGA1 with ab202070 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on BxPC-3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:
-ve control 1: ab202070 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
![Flow Cytometry - Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)](https://www.abcam.com/ps/products/251/ab251363/Images/ab251363-1-anti-hmga1-antibody-epr16649-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)This data was developed using ab202070, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HMGA1 with ab202070 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. -
![Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)](https://www.abcam.com/ps/products/251/ab251363/Images/ab251363-4-benefits-of-recombinant-antibodies.png)
Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (ab251363)
