Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HMGA1 antibody [EPR22421-101] - BSA and Azide free (ab255277)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22421-101] to HMGA1 - BSA and Azide free
  • Suitable for: ChIP, WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-HMGA1 antibody [EPR22421-101] - BSA and Azide free
    See all HMGA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22421-101] to HMGA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HT-1080, NCCIT, HCT116 whole cell lysate. Human testis tissue lysate and Human colon cancer tissue lysate. IP: HCT116 whole cell lysate. IHC-P: Human testis tissue. Human pancreas cancer and lung cancer tissue. ICC/IF: NCCIT cells. Flow Cyt: NCCIT cells. ChIP: EL4 cells.

  • General notes

    ab255277 is the carrier-free version of ab252930. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255277 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • ChIP - Anti-HMGA1 antibody [EPR22421-101] - BSA and Azide free (ab255277)
    ChIP - Anti-HMGA1 antibody [EPR22421-101] - BSA and Azide free (ab255277)

    Chromatin was prepared from EL4 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min.

    The ChIP was performed with 25 µg of chromatin, 5 µg of ab252930 (red) or 5 μg of rabbit normal IgG (gray) was added to 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (sybr green approach).

    Primers and probes are located in the first kb of the transcribed region.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252930).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [EPR22421-101] - BSA and Azide free (ab255277)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [EPR22421-101] - BSA and Azide free (ab255277)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling HMGA1 with ab252930 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in germ cells of human testis (PMID:17935122) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252930).

  • Immunoprecipitation - Anti-HMGA1 antibody [EPR22421-101] - BSA and Azide free (ab255277)
    Immunoprecipitation - Anti-HMGA1 antibody [EPR22421-101] - BSA and Azide free (ab255277)

    HMGA1 was immunoprecipitated from 0.35 mg HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate with ab252930 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab252930 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

    Lane 1: HCT 116 whole cell lysate 10 µg (Input).
    Lane 2: ab252930 IP in HCT 116 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252930 in HCT 116 whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 40 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252930).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [EPR22421-101] - BSA and Azide free (ab255277)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [EPR22421-101] - BSA and Azide free (ab255277)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue labeling HMGA1 with ab252930 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human pancreas cancer (PMID: 18473350) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252930).

  • Immunocytochemistry/ Immunofluorescence - Anti-HMGA1 antibody [EPR22421-101] - BSA and Azide free (ab255277)
    Immunocytochemistry/ Immunofluorescence - Anti-HMGA1 antibody [EPR22421-101] - BSA and Azide free (ab255277)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells labeling HMGA1 with ab252930 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in NCCIT cell line. Low expression cell line: MCF7?PMID: 12640109). The nuclear counter stain is DAPI (blue). Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).

    The negative control is the secondary antibody only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252930).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [EPR22421-101] - BSA and Azide free (ab255277)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [EPR22421-101] - BSA and Azide free (ab255277)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling HMGA1 with ab252930 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human lung cancer (PMID:25344216) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252930).

  • Flow Cytometry - Anti-HMGA1 antibody [EPR22421-101] - BSA and Azide free (ab255277)
    Flow Cytometry - Anti-HMGA1 antibody [EPR22421-101] - BSA and Azide free (ab255277)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell, Left) / NCCIT (Human pluripotent embryonic carcinoma epithelial cell, Right) cell lines labeling HMGA1 with ab252930 at 1/600 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Weak expression control: MCF7 (PMID:12640109).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252930).
  • Anti-HMGA1 antibody [EPR22421-101] - BSA and Azide free (ab255277)
    Anti-HMGA1 antibody [EPR22421-101] - BSA and Azide free (ab255277)

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