Human VTA1 ELISA Kit (ab222878)
Key features and details
- Sensitivity: 0.25 ng/ml
- Range: 0.391 ng/ml - 25 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human VTA1 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 4.4% Inter-assay Sample n Mean SD CV% Plasma 10.3% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.25 ng/ml -
Range
0.391 ng/ml - 25 ng/ml -
Recovery
98 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Rabbit, Cow, Dog, Pig, Monkey -
Product overview
The Human VTA1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab222878) is designed for detection of human VTA1 in plasma, serum, and cell culture samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures human VTA1 in less than 4 hours. A polyclonal antibody specific for human VTA1 has been pre-coated onto a 96-well microplate with removable strips. VTA1 in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for VTA1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
Vacuolar protein sorting-associated protein VTA1 homolog (VTA1), also known as dopamine-responsive gene 1 protein (DRG-1), LYST-interacting protein 5 (LIP5), and SKD1-binding protein 1 (SBP1), is predominantly a cytosolic protein. VTA1 consists of 307 amino acids with a predicted molecular mass of 34 kDa. It is involved in the endosomal multivesicular body (MVB) formation and trafficking. The MVB is an endosomal compartment that serves to sort membrane proteins destined for degradation or routing to the lysosome. These membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids, are internalized into intraluminal vesicles that are generated by the invagination and scission from the limiting membrane of the endosome. The contents of the MVB are then transferred to lysosomes. VTA1 interacts with charged multivesicular body protein 5 (CHMP5) to form stable complexes in the cytoplasm, binds well to CHMP1B, CHMP2A, and CHMP3 proteins for MVB sorting, and is required for immunodeficiency virus type 1 HIV particle release.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 20ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml Anti-Human VTA1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Human VTA1 1 x 120µl Chromogen Substrate 1 x 7ml Human VTA1 Standard (Lyophilized) 1 vial Sealing Tapes 1 x 3 units Stop Solution 1 x 11ml -
Research areas
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Function
Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. Involved in the sorting and down-regulation of EGFR (By similarity). Involved in HIV-1 budding. -
Sequence similarities
Belongs to the VTA1 family. -
Cellular localization
Cytoplasm. Endosome membrane. - Information by UniProt
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Alternative names
- C6orf55
- Chromosome 6 open reading frame 55
- Dopamine responsive protein DRG 1
see all -
Database links
- Entrez Gene: 51534 Human
- Omim: 610902 Human
- SwissProt: Q9NP79 Human
- Unigene: 431367 Human
Images
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Human VTA1 standard curve
Example of human VTA1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.