Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7668] to BANF1/BAF - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
  • Reacts with: Dog, Human

Overview

  • Product name

    Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free
    See all BANF1/BAF primary antibodies

  • Description

    Rabbit monoclonal [EPR7668] to BANF1/BAF - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

    See IHC antigen retrieval protocols.

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Dog, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: NIH/3T3 cells. Flow Cyt: SH-SY5Y and NIH/3T3 cells. WB: PC-12, Jurkat, U-87 MG, HeLa and NIH/3T3 cell lysate. IHC-P: Human astrocytoma, colon carcinoma, and glioma tissues.

  • General notes

    ab240953 is the carrier-free version of ab129184 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab240953 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as BANF1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Dissociation constant (KD)

    KD = 2.18 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR7668

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
    Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)

    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling BANF1/BAF with Purified ab129184 at 1:100 dilution (7.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling BANF1/BAF with Purified ab129184 at 1:100 dilution (0.78 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)

  • OI-RD Scanning - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
    OI-RD Scanning - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)

    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)

    ab129184, at a 1/100 dilution, staining BANF1/BAF in paraffin embedded human colonic carcinoma tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
    Flow Cytometry - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling BANF1/BAF (red) with ab129184 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)

  • Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
    Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryo fibroblast cells) cells labeling BANF1/BAF with ab129184 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing mainly nuclear with cytoplasmic staining on NIH/3T3 cell line. The nuclear counterstain is DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)

  • Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
    Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma) cells labeling BANF1/BAF with ab129184 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing mainly nuclear with weakly cytoplasmic staining on Hela cell line. The nuclear counterstain is DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953) This image is courtesy of an anonymous Abreview

    ab129184 staining BANF1/BAF in MDCK (Canine kidney cell line) cell pellets in paraffin by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Cell pellets were fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 1% serum for 2 hours at room temperature; antigen retrieval was by heat mediation in Tris/EDTA pH 9. Samples were incubated with primary antibody (1/100 in 1% BSA + 1% FBS) for 16 hours. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)

  • Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
    Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)

© 2021 Astana Biomed Group LLP. All rights reserved.