Anti-Beta TRCP/HOS antibody [4C5D8] (ab233638)
![Anti-Beta TRCP/HOS antibody [4C5D8] (ab233638)](https://www.abcam.com/ps/products/233/ab233638/Images/ab233638-306965-ab2336381.jpg "Anti-Beta TRCP/HOS antibody [4C5D8] (ab233638)")
Key features and details
- Mouse monoclonal [4C5D8] to Beta TRCP/HOS
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Beta TRCP/HOS antibody [4C5D8]
See all Beta TRCP/HOS primary antibodies -
Description
Mouse monoclonal [4C5D8] to Beta TRCP/HOS -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human Beta TRCP/HOS aa 24-151.
Database link: Q9Y297 -
Positive control
- WB: Beta TRCP/HOS (aa 24-151)-hIgGFc transfected HEK-293 cell lysate; Ramos, MCF7 and K562 cell lysates; Human beta TCRP (aa 24-151) recombinant protein. Flow Cyt: HeLa cells. IHC-P: Human esophageal cancer and rectum cancer tissues.
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General notes
This product was previously labelled as Beta TRCP
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
4C5D8 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Beta TRCP/HOS antibody [4C5D8] (ab233638)Anti-Beta TRCP/HOS antibody [4C5D8] (ab233638) at 1/500 dilution + Human Beta TRCP/HOS (aa 24-151) recombinant protein
Predicted band size: 68 kDaExpected MW is 40.2 kDa.
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![Western blot - Anti-Beta TRCP/HOS antibody [4C5D8] (ab233638)](https://www.abcam.com/ps/products/233/ab233638/Images/ab233638-306964-anti-beta-trcp-antibody-4c5d8-western-blot.jpg)
Western blot - Anti-Beta TRCP/HOS antibody [4C5D8] (ab233638)All lanes : Anti-Beta TRCP/HOS antibody [4C5D8] (ab233638) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : beta TCRP/HOS (aa 24-151)-hIgGFc transfected HEK-293 cell lysate
Predicted band size: 68 kDa
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![Western blot - Anti-Beta TRCP/HOS antibody [4C5D8] (ab233638)](https://www.abcam.com/ps/products/233/ab233638/Images/ab233638-306963-anti-beta-trcp-antibody-4c5d8-western-blot.jpg)
Western blot - Anti-Beta TRCP/HOS antibody [4C5D8] (ab233638)All lanes : Anti-Beta TRCP/HOS antibody [4C5D8] (ab233638) at 1/500 dilution
Lane 1 : Ramos (human Burkitt's lymphoma cell line) cell lysate
Lane 2 : NCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Predicted band size: 68 kDa
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![Flow Cytometry - Anti-Beta TRCP/HOS antibody [4C5D8] (ab233638)](https://www.abcam.com/ps/products/233/ab233638/Images/ab233638-306962-anti-beta-trcp-antibody-4c5d8-flow-cytometry.jpg)
Flow Cytometry - Anti-Beta TRCP/HOS antibody [4C5D8] (ab233638)Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Beta TRCP/HOS with ab233638 at 1/200 dilution (green) compared with a negative control (red).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beta TRCP/HOS antibody [4C5D8] (ab233638)](https://www.abcam.com/ps/products/233/ab233638/Images/ab233638-306961-anti-beta-trcp-antibody-4c5d8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beta TRCP/HOS antibody [4C5D8] (ab233638)Paraffin-embedded human esophageal cancer tissue stained for Beta TRCP/HOS using ab233638 at 1/200 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beta TRCP/HOS antibody [4C5D8] (ab233638)](https://www.abcam.com/ps/products/233/ab233638/Images/ab233638-306960-anti-beta-trcp-antibody-4c5d8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beta TRCP/HOS antibody [4C5D8] (ab233638)Paraffin-embedded human rectum cancer tissue stained for Beta TRCP/HOS using ab233638 at 1/200 dilution in immunohistochemical analysis.
