HRP Anti-UQCRFS1/RISP antibody [5A5] (ab198392)
![HRP Anti-UQCRFS1/RISP antibody [5A5] (ab198392)](https://www.abcam.com/ps/products/198/ab198392/Images/ab198392-238422-WBab1983921.jpg "HRP Anti-UQCRFS1/RISP antibody [5A5] (ab198392)")
Key features and details
- HRP Mouse monoclonal [5A5] to UQCRFS1/RISP
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-UQCRFS1/RISP antibody [5A5]
See all UQCRFS1/RISP primary antibodies -
Description
HRP Mouse monoclonal [5A5] to UQCRFS1/RISP -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Other Immunogen Type corresponding to Cow UQCRFS1/RISP. Bovine Complex III.
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Positive control
- WB: Human heart mitochondria lysate.
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General notes
Previously labelled as UQCRFS1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
5A5 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-UQCRFS1/RISP antibody [5A5] (ab198392)HRP Anti-UQCRFS1/RISP antibody [5A5] (ab198392) at 1/5000 dilution + Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198392 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
