Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16289] to UQCRFS1/RISP - C-terminal
  • Suitable for: IHC-P, ICC/IF, IP, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal
    See all UQCRFS1/RISP primary antibodies

  • Description

    Rabbit monoclonal [EPR16289] to UQCRFS1/RISP - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, Raji, 293, PC12, mouse brain and Human fetal heart lysates; Human kidney tissue; HepG2 and HeLa cells.

  • General notes

    Previously labelled as UQCRFS1.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)
    Western blot - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)
    All lanes : Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079) at 1/1000 dilution

    Lane 1 : PC12 cell lysate
    Lanes 2-3 : Mouse brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 30 kDa

  • Immunoprecipitation - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)
    Immunoprecipitation - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)

    Western blot analysis of immunoprecipitation pellet from Human fetal heart lysate immunoprecipitated using ab191079 at 1/90 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Flow Cytometry - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)
    Flow Cytometry - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)

    Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling UQCRFS1/RISP with ab191079 at 1/280 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)
    Immunocytochemistry/ Immunofluorescence - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)

    Immunofluorescent analysis of HepG2 cells (4% Paraformaldehyde-fixed; 0.1% tritonX-100 permeabilized) labeling UQCRFS1/RISP with ab191079 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue). Negative controls also shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)


    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling UQCRFS1/RISP with ab191079 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)
    Western blot - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)
    All lanes : Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 30 kDa

  • Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)
    Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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