Anti-E1B-AP5 antibody [EP2633Y] (ab68480)
![Anti-E1B-AP5 antibody [EP2633Y] (ab68480)](https://www.abcam.com/ps/products/68/ab68480/Images/ab68480-397737-anti-e1b-ap5-antibody-ep2633y-western-blot-human-knockout-lysate.jpg "Anti-E1B-AP5 antibody [EP2633Y] (ab68480)")
Key features and details
- Rabbit monoclonal [EP2633Y] to E1B-AP5
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-E1B-AP5 antibody [EP2633Y]
See all E1B-AP5 primary antibodies -
Description
Rabbit monoclonal [EP2633Y] to E1B-AP5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human E1B-AP5 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: Raji, HEK293T and HeLa whole cell lysates. IHC-P: Human brain tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as HNRPUL1.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2633Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-E1B-AP5 antibody [EP2633Y] (ab68480)All lanes : Anti-E1B-AP5 antibody [EP2633Y] (ab68480) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : HNRNPUL1 knockout HEK293T cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 96 kDa
Observed band size: 96 kDaLanes 1-3: Merged signal (red and green). Green - ab68480 observed at 96 kDa. Red - loading control ab8245 observed at 36 kDa.
ab68480 Anti-E1B-AP5 antibody [EP2633Y] was shown to specifically react with E1B-AP5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266142 (knockout cell lysate ab257993) was used. Wild-type and E1B-AP5 knockout samples were subjected to SDS-PAGE. ab68480 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1B-AP5 antibody [EP2633Y] (ab68480)](https://www.abcam.com/ps/products/68/ab68480/Images/ab68480-82777-anti-e1b-ap5-antibody-ep2633y-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1B-AP5 antibody [EP2633Y] (ab68480)ab68480 at 1/250 dilution staining E1B-AP5 in human brain by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-E1B-AP5 antibody [EP2633Y] (ab68480)](https://www.abcam.com/ps/products/68/ab68480/Images/ab68480-82779-anti-e1b-ap5-antibody-ep2633y-western-blot.gif)
Western blot - Anti-E1B-AP5 antibody [EP2633Y] (ab68480)Anti-E1B-AP5 antibody [EP2633Y] (ab68480) at 1/50000 dilution + HeLa cell lysate at 10 µg
Secondary
goat anti-rabbit, HRP conjugated at 1/2000 dilution
Predicted band size: 96 kDa
Observed band size: 96 kDa
Additional bands at: 125 kDa. We are unsure as to the identity of these extra bands.
