Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16288] to UQCRFS1/RISP
  • Suitable for: WB, IP, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-UQCRFS1/RISP antibody [EPR16288]
    See all UQCRFS1/RISP primary antibodies

  • Description

    Rabbit monoclonal [EPR16288] to UQCRFS1/RISP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, Raji, 293, Human fetal heart, mouse brain, mouse kidney, mouse heart, rat brain, rat heart, rat kidney, RAW264.7 and NIH3T3 lysates; mouse stomach, Human ovarian adenocarcinoma and Human kidney tissues; HeLa cells.

  • General notes

    Previously labelled as UQCRFS1.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078)
    Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078)
    All lanes : Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : Raji lysates
    Lane 3 : 293 lysates
    Lane 4 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078)
    Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078)
    All lanes : Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078) at 1/1000 dilution

    Lane 1 : mouse brain lysate
    Lane 2 : mouse heart lysate
    Lane 3 : mouse kidney lysate
    Lane 4 : rat brain lysate
    Lane 5 : rat heart lysate
    Lane 6 : rat kidney lysate
    Lane 7 : RAW264.7 lysate
    Lane 8 : NIH3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078)
    Immunoprecipitation - Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078)

    Western blot analysis of UQCRFS1/RISP in immunoprecipitation pellets from Human fetal lysate. ab191078 used at 1/60 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078)
    Immunocytochemistry/ Immunofluorescence - Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078)

    Immunofluorescent analysis of methanol-fixed HeLa cells labeling UQCRFS1/RISP with ab191078 at 1/50, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 and DAPI staining (blue). Negative controls also shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling UQCRFS1/RISP with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078)

    Immunohistochemical analysis of paraffin-embedded Human ovarian adenocarcinoma tissue labeling UQCRFS1/RISP with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078)

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling UQCRFS1/RISP with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078)
    Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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