Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16545] to NLP1 - BSA and Azide free
  • Suitable for: IP, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NLP1 antibody [EPR16545] - BSA and Azide free
    See all NLP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16545] to NLP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251143 is the carrier-free version of ab192609. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251143 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)
    Immunocytochemistry - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)

    This data was developed using ab192609, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling NLP1 with ab192609 at 1/100 dilution (6.3μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue). Negative control also shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)

    This data was developed using ab192609, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling NLP1 with ab192609 at 1/100 dilution (6 μg/ml); followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)

    This data was developed using ab192609, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling NLP1 with ab192609 at 1/100 dilution (6 μg/ml); followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)
    This data was developed using ab192609, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling NLP1 with ab192609 at 1/100 dilution (6 μg/ml); followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)
    Western blot - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)
    All lanes : Anti-NLP1 antibody [EPR16545] (ab192609) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH/3T3 cell lysate
    Lane 5 : Mouse brain tissue lysate
    Lane 6 : Mouse heart tissue lysate
    Lane 7 : Mouse kidney tissue lysate
    Lane 8 : Mouse spleen tissue lysate
    Lane 9 : Rat brain tissue lysate
    Lane 10 : Rat heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa



    This data was developed using ab192609, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)
    Western blot - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)
    Anti-NLP1 antibody [EPR16545] (ab192609) at 1/1000 dilution + Human fetal liver tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 45 kDa



    This data was developed using ab192609, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)
    Western blot - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)
    All lanes : Anti-NLP1 antibody [EPR16545] (ab192609) at 1/10000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 45 kDa



    This data was developed using ab192609, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)
    Immunoprecipitation - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)
    This data was developed using ab192609, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab192609 at 1/40 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
  • Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)
    Anti-NLP1 antibody [EPR16545] - BSA and Azide free (ab251143)

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